中国药师
中國藥師
중국약사
CHINA PHARMACIST
2014年
4期
686-687
,共2页
高效液相色谱法%洗剂%甲氧沙林%醋酸地塞米松%含量测定
高效液相色譜法%洗劑%甲氧沙林%醋痠地塞米鬆%含量測定
고효액상색보법%세제%갑양사림%작산지새미송%함량측정
HPLC%Lotions%Methoxsalen%Dexamethasone acetate%Determination
目的：建立高效液相色谱法测定复方甲氧沙林洗剂中甲氧沙林和醋酸地塞米松的含量。方法：采用HPLC法,色谱柱：Sunfire C18(250 mm ×4.6 mm,5μm ),流动相：乙腈-水(60∶40),流速1.0 ml·min-1,检测波长：240 nm,柱温：30℃,进样量：20μl。结果：甲氧沙林和醋酸地塞米松分别在12.71~101.68 mg·L-1(r =0.9999)和10.03~80.25 mg·L-1(r =0.9997)范围内线性关系良好,平均回收率分别为99.32%(RSD=0.11%,n=9)和99.63%(RSD=0.20%,n=9)。结论：该法简便、准确、重复性好,可用于复方甲氧沙林洗剂中甲氧沙林和醋酸地塞米松的含量测定。
目的：建立高效液相色譜法測定複方甲氧沙林洗劑中甲氧沙林和醋痠地塞米鬆的含量。方法：採用HPLC法,色譜柱：Sunfire C18(250 mm ×4.6 mm,5μm ),流動相：乙腈-水(60∶40),流速1.0 ml·min-1,檢測波長：240 nm,柱溫：30℃,進樣量：20μl。結果：甲氧沙林和醋痠地塞米鬆分彆在12.71~101.68 mg·L-1(r =0.9999)和10.03~80.25 mg·L-1(r =0.9997)範圍內線性關繫良好,平均迴收率分彆為99.32%(RSD=0.11%,n=9)和99.63%(RSD=0.20%,n=9)。結論：該法簡便、準確、重複性好,可用于複方甲氧沙林洗劑中甲氧沙林和醋痠地塞米鬆的含量測定。
목적：건립고효액상색보법측정복방갑양사림세제중갑양사림화작산지새미송적함량。방법：채용HPLC법,색보주：Sunfire C18(250 mm ×4.6 mm,5μm ),류동상：을정-수(60∶40),류속1.0 ml·min-1,검측파장：240 nm,주온：30℃,진양량：20μl。결과：갑양사림화작산지새미송분별재12.71~101.68 mg·L-1(r =0.9999)화10.03~80.25 mg·L-1(r =0.9997)범위내선성관계량호,평균회수솔분별위99.32%(RSD=0.11%,n=9)화99.63%(RSD=0.20%,n=9)。결론：해법간편、준학、중복성호,가용우복방갑양사림세제중갑양사림화작산지새미송적함량측정。
Objective:To establish an HPLC method for the determination of methoxsalen and dexamethasone acetate in compound methoxsalen lotions. Methods:An HPLC method was used. The determination was performed on a Sunfire C18 (250 mm × 4. 6 mm,5μm ) column with the mobile phase of acetonitrile-water ( 60∶40 ) at the flow rate of 1. 0 ml·min-1 . The column temperature was kept at 30℃, the detection wavelength was set at 240nm and the injection volume was 20μl. Results:Methoxsalen and dexamethasone acetate showed good linear relationship within the range of 12.71-101.68 mg·L-1(r=0.999 9) and 10.03-80.25 mg·L-1(r=0. 999 7) with the average recovery of 99. 32%(RSD=0. 11%, n=9 ) and 99. 63%(RSD=0. 20%,n=9) , respectively. Conclu-sion:The method is simple, accurate and repeatable, and suitable for the determination of methoxsalen and dexamethasone acetate in compound methoxsalen lotions.
