汕头大学学报：自然科学版
汕頭大學學報：自然科學版
산두대학학보：자연과학판
Journal of Shantou University(Natural Science Edition)
2012年
3期
61-64
,共4页
肝癌衍生生长因子%脊髓损伤%免疫组化
肝癌衍生生長因子%脊髓損傷%免疫組化
간암연생생장인자%척수손상%면역조화
Hepatoma-derived growth factor%spinal cord injury (SCI)%immunhistochemical
目的：观察大鼠受损伤脊髓组织中肝癌衍生生长因子（Hepatoma—derivedgrowthfactor，HDGF）表达的变化．方法：将40只成年大白鼠随机分成对照组（A组）和脊髓损伤组（B组），每组20只．脊髓损伤组采用改良的Allen’S打靶法制备，并于术后24h处死大鼠，取损伤脊髓节段进行免疫组织化学染色．观察HDGF在正常对照组以及脊髓损伤后表达情况．以表达指数作为统计学指标，采用x2检验分析各组间的变化．结果：HDGF主要表达在神经细胞核中．15例损伤脊髓标本表现为HDGF表达指数为Ⅱ组，对照组中表达指数为Ⅱ组仅4只（P〈0．05）．结论：HDGF高表达于损伤脊髓组织中，提示HDGF可能参与脊髓损伤的修复．
目的：觀察大鼠受損傷脊髓組織中肝癌衍生生長因子（Hepatoma—derivedgrowthfactor，HDGF）錶達的變化．方法：將40隻成年大白鼠隨機分成對照組（A組）和脊髓損傷組（B組），每組20隻．脊髓損傷組採用改良的Allen’S打靶法製備，併于術後24h處死大鼠，取損傷脊髓節段進行免疫組織化學染色．觀察HDGF在正常對照組以及脊髓損傷後錶達情況．以錶達指數作為統計學指標，採用x2檢驗分析各組間的變化．結果：HDGF主要錶達在神經細胞覈中．15例損傷脊髓標本錶現為HDGF錶達指數為Ⅱ組，對照組中錶達指數為Ⅱ組僅4隻（P〈0．05）．結論：HDGF高錶達于損傷脊髓組織中，提示HDGF可能參與脊髓損傷的脩複．
목적：관찰대서수손상척수조직중간암연생생장인자（Hepatoma—derivedgrowthfactor，HDGF）표체적변화．방법：장40지성년대백서수궤분성대조조（A조）화척수손상조（B조），매조20지．척수손상조채용개량적Allen’S타파법제비，병우술후24h처사대서，취손상척수절단진행면역조직화학염색．관찰HDGF재정상대조조이급척수손상후표체정황．이표체지수작위통계학지표，채용x2검험분석각조간적변화．결과：HDGF주요표체재신경세포핵중．15례손상척수표본표현위HDGF표체지수위Ⅱ조，대조조중표체지수위Ⅱ조부4지（P〈0．05）．결론：HDGF고표체우손상척수조직중，제시HDGF가능삼여척수손상적수복．
AIM： To study the change of expression of Hepatoma-derived growth factor （HDGF） after spinal cord injury （SCI） in adult rats. METHODS： 40 adult Sprague- Dawley rats were randomly divided into two groups, 20 rats in every group. Adult rats were given contusion SCI at the T9-10 vertebrae level with a weight-drop impactor （10g weight dropped 25.0ram） in B group. Other rats in A group were as control. The expression of HDGF after SCI was assessed using immunohistochemical staining. HDGF expression index （EI） in neuron was analyzed. RESULTS： HDGF expression was observed in all cases, and the expression of HDGF in nerve cells was mainly located in the nuclear. 75.0%（15/20） HDGF E1 levels 2 for nuclei was in B group, and only 20.0%（4 /20） HDGF EI 2 was in A group. The expression of HDGF was significantly higher in the SCI group than in the control group （P 〈 0.05）. CONCLUSION： Over-expressed （HDGF） after SCI suggests HDGF might be involved in the preserve and repair of SCI.
