CAJ | 학술논문

建立高效液相色谱法检测白龙散、黄连解毒散、苍术香连散等7种兽用中药散剂中非法添加利巴韦林的方法。用十八烷基硅烷键合硅胶色谱柱，以水（用稀硫酸调节pH值至2．5±0．1）为流动相，检测波长207nm，流速1．0mL／min，进样量20μL。结果显示，利巴韦林的线性范围为1．016—20．32μg／mL，线性方程为Y=5．68×10X-5．61×10^-2（r=0．9999，n=6），回收率为81．28％～119．46％，RSD为0．88％～1．65％。本方法准确可靠，适用于兽用中药散剂中非法添加利巴韦林的检测。
건립고효액상색보법검측백룡산、황련해독산、창술향련산등7충수용중약산제중비법첨가리파위림적방법。용십팔완기규완건합규효색보주，이수（용희류산조절pH치지2．5±0．1）위류동상，검측파장207nm，류속1．0mL／min，진양량20μL。결과현시，리파위림적선성범위위1．016—20．32μg／mL，선성방정위Y=5．68×10X-5．61×10^-2（r=0．9999，n=6），회수솔위81．28％～119．46％，RSD위0．88％～1．65％。본방법준학가고，괄용우수용중약산제중비법첨가리파위림적검측。
To establish a method for the determination of ribavirin added illegally in veterinary Chinese medicine powders by HPLC,a reverse-phase C18 column was used with the mobile phase of water （adjusted the pH value with dilute sulphuric acid to 2.5＋0.1 ） at 207 nm, and the flow rate was 1.0 mL/min. The results demonstrated that the linearity range of ribavirin was 1.016-20.32μg/mL, the linear equation was Y= 5.68×10 X - 5.61×10-2（r= 0..999 9, n=6 ）. The recovery rate was 81.28%- 119.46% and RSD was 0.88%-1.65%. It is indicated that this method is accurate, reliable and suitable for the determination of ribavirin added illegally in veterinary Chinese medicine powders such as Bailong San, Cartgzhuxianglian San and Huanglianjiedu San, etc.