CAJ | 학술논문

目的：探讨黄芪复方剂提取物总黄酮（EFA）对CCh致大鼠肝氧化损伤的保护作用及机制。方法：将雄性sD大鼠60只随机分成4组，即灌胃灭菌生理盐水空白对照组和模型组、联苯双酯（100mg／kg）溶液阳性药物对照组、黄芪复方剂提取物总黄酮（200mg／kg）组。除空白对照组腹腔注射等量生理盐水外，其余各组腹腔注射四氯化碳玉米油（2mL／kg）造成大鼠肝氧化损伤模型，后分别于12、24h将相应大鼠处死，检测血清中ALT、AST、ALB含量与肝组织中MDA、S01）、GSH—Px活性。结果：与模型组比较，经黄酮药物组预防性治疗后，AST、ALT转氨酶明显降低（P〈O．05），而ALB含量增加不明显；同时肝脏中MDA含量明显降低（P〈0．05），SOD、GSH—Px活性接近空白组，且明显高于模型组（P〈0．01）。结论：EFA对四氯化碳造成的大鼠肝氧化损伤具有保护作用。
목적：탐토황기복방제제취물총황동（EFA）대CCh치대서간양화손상적보호작용급궤제。방법：장웅성sD대서60지수궤분성4조，즉관위멸균생리염수공백대조조화모형조、련분쌍지（100mg／kg）용액양성약물대조조、황기복방제제취물총황동（200mg／kg）조。제공백대조조복강주사등량생리염수외，기여각조복강주사사록화탄옥미유（2mL／kg）조성대서간양화손상모형，후분별우12、24h장상응대서처사，검측혈청중ALT、AST、ALB함량여간조직중MDA、S01）、GSH—Px활성。결과：여모형조비교，경황동약물조예방성치료후，AST、ALT전안매명현강저（P〈O．05），이ALB함량증가불명현；동시간장중MDA함량명현강저（P〈0．05），SOD、GSH—Px활성접근공백조，차명현고우모형조（P〈0．01）。결론：EFA대사록화탄조성적대서간양화손상구유보호작용。
Objective： To investigate the effect and mechaism of flavo.oid sact from Radi Astragali bifendate （100 mg/kg） by gastrogavage twice daily, respectively. After 12 and 24 hours, the contents of ALT, AST and ALB in serum were determined, and the level of hepatic MDA, SOD and GSH-Px were also detected. Results： After treatment with flavonoid extract, the contents of ALT, AST and hepatic MDA in rats were significantly decreased（P〈0.05）, while the contents of ALB had no changes compared with that of the model group. Moreover, the level of SOD and GSH-Px in treatment group were all approximated to that of the normal control, but higher than that of the model group. Conclusion： Flavonoid extract from Radix Astragali compound preparation could protect rats from acute hepatic injury induced by CC14.