国际中医中药杂志
國際中醫中藥雜誌
국제중의중약잡지
INTERNATIONAL JOURNAL OF TRIDITIONAL CHINESE MEDICINE
2013年
7期
613-615
,共3页
萹蓄%杨梅苷%槲皮苷%高效液相色谱法
萹蓄%楊梅苷%槲皮苷%高效液相色譜法
편축%양매감%곡피감%고효액상색보법
Polygonum aviculare L.%Myricetrin%Quercitroside%RP-HPLC
目的 采用反相高效液相色谱法(HPLC)分析萹蓄中杨梅苷和槲皮苷的含量.方法 以Agilent LC-C18 (250 mm×4.6 mm,5μm)色谱柱,以乙腈-0.1％磷酸溶液(18∶82)作为流动相,流速为1.0 ml/min,柱温:30℃；检测波长:256 nm.结果 杨梅苷和槲皮苷分别在1.22～24.30 tg/ml和0.77～15.40 μg/ml范围内有良好的线性关系,平均加样回收率分别为98.9％、99.6％,RSD分别为1.11％、1.09％.结论 本方法可用于萹蓄中杨梅苷和槲皮苷的定量分析.
目的 採用反相高效液相色譜法(HPLC)分析萹蓄中楊梅苷和槲皮苷的含量.方法 以Agilent LC-C18 (250 mm×4.6 mm,5μm)色譜柱,以乙腈-0.1％燐痠溶液(18∶82)作為流動相,流速為1.0 ml/min,柱溫:30℃；檢測波長:256 nm.結果 楊梅苷和槲皮苷分彆在1.22～24.30 tg/ml和0.77～15.40 μg/ml範圍內有良好的線性關繫,平均加樣迴收率分彆為98.9％、99.6％,RSD分彆為1.11％、1.09％.結論 本方法可用于萹蓄中楊梅苷和槲皮苷的定量分析.
목적 채용반상고효액상색보법(HPLC)분석편축중양매감화곡피감적함량.방법 이Agilent LC-C18 (250 mm×4.6 mm,5μm)색보주,이을정-0.1％린산용액(18∶82)작위류동상,류속위1.0 ml/min,주온:30℃；검측파장:256 nm.결과 양매감화곡피감분별재1.22～24.30 tg/ml화0.77～15.40 μg/ml범위내유량호적선성관계,평균가양회수솔분별위98.9％、99.6％,RSD분별위1.11％、1.09％.결론 본방법가용우편축중양매감화곡피감적정량분석.
Objective To establish the RP-HPLC method for the determination of myricetrin and quercitroside in Polygonum aviculare L.Methods The Agilent LC-C18 (250 mm×4.6 mm,5 μm) column was used,the mobile phase consisted of acetonitrile:0.1％ H3PO4(18 ∶ 82),the flow rate was 1.0 ml/min,the column temperature was 30℃ the detecting wavelength was at 256 nm.Results The calibration curve was linear within a range of 1.22～24.30 μg/ml and 0.77～15.40 μg/ml,the average recovery of this method was 98.9％,99.6％ and the RSD was 1.11％,1.09％,for the myricetrin and quercitroside respectively.Conclusion The method is simple,repeatable and accurate.It can be applied in quantitative determination of myricetrin and quercitroside in Polygonum aviculare L..
