CAJ | 학술논문

以盐酸小檗碱为对照品，采用紫外分光光度法在410nm处测定赛北紫堇中总生物碱含量。结果表明，测定在6．48～38．88μg／mL范围内线性关系良好，回归方程：Y=0．00825x-0．00200，R2=0．99979（n=7），平均加标回收率为101．70％，相对标准偏差（RSD）为3．19％。实验结果表明，用紫外分光光度法测定赛北紫堇总生物碱含量具有稳定性好、方法简便的优点。
이염산소벽감위대조품，채용자외분광광도법재410nm처측정새북자근중총생물감함량。결과표명，측정재6．48～38．88μg／mL범위내선성관계량호，회귀방정：Y=0．00825x-0．00200，R2=0．99979（n=7），평균가표회수솔위101．70％，상대표준편차（RSD）위3．19％。실험결과표명，용자외분광광도법측정새북자근총생물감함량구유은정성호、방법간편적우점。
The determination was conducted by uhraviolet spectrophotometry photometer under 410 nm ultraviolet radiatio, while the Berberine hydroehloride was selected as contrast substance. The results showed： the linearity relation between absorbency and content is good when the content is 6.48 - 38.88μg/ mL, and the regression equation is y = 0. 00825x - 0.00200, R2 = 0.99979 （ n = 7 ） , average complementary post recovery rate is 101.70. RSD = 3.19%. Ultraviolet spectrophotometry is simple and quick method to de- termine the content of the total alkaloids in Corydalis impatiens with good stability.