CAJ | 학술논문

流行病学调查显示：糖尿病患者比正常人得帕金森病的几率要高83％以上。尤其早期糖尿病，在没有其他并发症的时候，其患帕金森的几率是正常人的7倍。而在帕金森病的晚期，有一个特征就是黑质纹状体通路的多巴胺含量减少。本研究以SH—SY5Y神经母细胞瘤作为多巴胺能神经元的细胞模型，首先用200mmol／L葡萄糖处理SH—SY5Y模型细胞48小时，用HPLC—EcD检测细胞内多巴胺的含量，发现SH—SY5Y细胞在200mmol／L的葡萄糖处理48dx时后，细胞内的多巴胺含量降低。本研究提示我们，在长期的高血糖条件下，细胞内会产生类似帕金森病的多巴胺减少的效应。
류행병학조사현시：당뇨병환자비정상인득파금삼병적궤솔요고83％이상。우기조기당뇨병，재몰유기타병발증적시후，기환파금삼적궤솔시정상인적7배。이재파금삼병적만기，유일개특정취시흑질문상체통로적다파알함량감소。본연구이SH—SY5Y신경모세포류작위다파알능신경원적세포모형，수선용200mmol／L포도당처리SH—SY5Y모형세포48소시，용HPLC—EcD검측세포내다파알적함량，발현SH—SY5Y세포재200mmol／L적포도당처리48dx시후，세포내적다파알함량강저。본연구제시아문，재장기적고혈당조건하，세포내회산생유사파금삼병적다파알감소적효응。
The epidemiologieal survey have shown that the diabetic patient suffering from Parkinson＇ s disease is more than 83% higher than normal persons. A feature of Parkinson＇s disease is the dopamine content decreasing in nigrostriatal pathway. In our studies, we use SH-SY5Y neuroblastoma as the dopaminergic neurons cell model. The SH-SY5Y cells were incubated with 200mmol / L glucose for 48 hours, and checked the dopamine content using HPLC-ECD. The research suggests that high-glucose could cause the reducing of dopamine content in SH-SY5Y ceils.