沈阳大学学报
瀋暘大學學報
침양대학학보
JOURNAL OF SHENYANG UNIVERSITY
2012年
4期
1-6
,共6页
宏基因组技术%文库构建%文库筛选%厌氧消化%微生物群落
宏基因組技術%文庫構建%文庫篩選%厭氧消化%微生物群落
굉기인조기술%문고구건%문고사선%염양소화%미생물군락
metagenomic technology%libraries construction%libraries screening%anaerobic digestion~microbial community
在介绍基因(组)富集、基因(组)DNA提取、文库构建及筛选等宏基因组技术的基础上,论述了宏基因组技术在厌氧消化研究中应用的重要性,认为该技术可成为研究微生物群落多样性的首选技术之一,有助于了解厌氧消化微生物的多样性、生态功能和动态变化,揭示厌氧消化微生物学机理,提高厌氧消化效能等.同时宏基因组技术为微生物资源的深层次开发利用提供了技术平台,并在工业、农业、海洋等领域具有广泛的应用前景.
在介紹基因(組)富集、基因(組)DNA提取、文庫構建及篩選等宏基因組技術的基礎上,論述瞭宏基因組技術在厭氧消化研究中應用的重要性,認為該技術可成為研究微生物群落多樣性的首選技術之一,有助于瞭解厭氧消化微生物的多樣性、生態功能和動態變化,揭示厭氧消化微生物學機理,提高厭氧消化效能等.同時宏基因組技術為微生物資源的深層次開髮利用提供瞭技術平檯,併在工業、農業、海洋等領域具有廣汎的應用前景.
재개소기인(조)부집、기인(조)DNA제취、문고구건급사선등굉기인조기술적기출상,논술료굉기인조기술재염양소화연구중응용적중요성,인위해기술가성위연구미생물군락다양성적수선기술지일,유조우료해염양소화미생물적다양성、생태공능화동태변화,게시염양소화미생물학궤리,제고염양소화효능등.동시굉기인조기술위미생물자원적심층차개발이용제공료기술평태,병재공업、농업、해양등영역구유엄범적응용전경.
The metagenomic technology is introduced, which is composed of enrichment of gene DNA, extraction of DNA, construction and screening of clone libraries, etc. Metagenomic technology can make it possible to enormously amplify the space of microbial resource utilization it has been broadly applied in industry, agriculture and ocean etc. This methodology is of great potential for use in anaerobic digestion research, it can be used to study microbial diversity in anaerobic digestor, enable to peep at more complete scenario of microbial communities and to better find out the spatial distribution and spatio-temporal dynamics of microorganisms in anaerobic digestor, it also can reveal the microbial mechanism of anaerobic digestion, enhance efficiency of digestion reactors. Metagenomie technology provides a technology platform for the deep-seated development and utilization of microbial resources it has broad prospects in the field of industrial, agricultural, marine and other applications.