CAJ | 학술논문

采用阻断ELISA方法对来自天峻县的327份牦牛血清进行了牛BVDV特异性抗体的检测,共检出231份血清阳性,血清阳性率为70.64%。其中：在135份野牦牛血清中检出110份阳性血清,阳性率为81.48%;在192份家牦牛血清中检出阳性121份,阳性率为63.02%。从231份ELISA检测阳性血清中随机抽取了20份,采用RT-PCR方法进行检测,结果共检出18份样品为BVDV/MV RT-PCR阳性,阴性血清未扩增出E0基因,RT-PCR方法检测结果与ELISA方法符合率为90%。
채용조단ELISA방법대래자천준현적327빈모우혈청진행료우BVDV특이성항체적검측,공검출231빈혈청양성,혈청양성솔위70.64%。기중：재135빈야모우혈청중검출110빈양성혈청,양성솔위81.48%;재192빈가모우혈청중검출양성121빈,양성솔위63.02%。종231빈ELISA검측양성혈청중수궤추취료20빈,채용RT-PCR방법진행검측,결과공검출18빈양품위BVDV/MV RT-PCR양성,음성혈청미확증출E0기인,RT-PCR방법검측결과여ELISA방법부합솔위90%。
327 serumsamplees were collected from yaks in Tianjun county in Qinghai Provence anddetected with block - ELISA and RT - PCR method. The result showed that 231 of them are positive, positive rate of serum is 70.64%. 110 positive serum has been detected in 135 serum samples of wild yaks, the positive rate is 81.48% ; 121 positive serum has been detected in 192 serum samples by ELISA Randomly and are detected by RT- PCR mehod, 18 is BVDV/MV RT - PCR positive, Eo gene are not amplified in negative serum. The results detected by RT - PCR and ELISA methvod has consistency of 90%.