CAJ | 학술논문

观察除草啶经口饲喂90 d对大鼠血液、尿液、脏器重,生化指标及病理组织学的变化。依据GB15670-1995《农药登记毒理学试验方法》对其进行了为期90 d的毒性实验。结果,试验期间各组动物一般情况良好,无死亡、厌食和其它异常行为;中、高剂量组雄、雌性大鼠周平均体重从第2周开始至实验结束均明显低于对照组（p〈0.05,p〈0.01）;高剂量组雄、雌性大鼠的食物消耗量明显低于对照组（p〈0.05）。血液学检查可见中剂量组雄性大鼠WBC明显低于对照组（p〈0.01）,但无剂量-反应关系。中、高剂量组雄性大鼠RBC和HGB均明显低于对照组（p〈0.01）;雌性大鼠的血常规检查未见明显改变。血清生化测定结果可见雄性大鼠中剂量组的BUN明显低于对照组（p〈0.05）,但无剂量-反应关系;中、高剂量组的TG明显低于对照组（p〈0.05、p〈0.01）,高剂量组的GLU明显低于对照组（p〈0.05）,提示受检样品对雄性大鼠糖代谢和脂代谢有一定影响;雌性大鼠中、高剂量组的ALP明显低于对照组（p〈0.05,p〈0.01）,高剂量的AST明显高于对照组（p〈0.05）、GLU明显低于对照组（p〈0.05）,提示受检样品对雌性大鼠肝功能、糖代谢有一定影响。中剂量的CHE明显高于对照组（p〈0.01）,但无剂量-反应关系。尿常规检查：各剂量组与对照组比较,各项检测指标无明显变化。脏器检查和脏器重量系数测定：试验结束进行大体解剖,各剂量组动物大体解剖结果均未见异常。脏器重量系数测定结果表明,中、高剂量组雄性大鼠脑、肝、肾、睾丸重量系数明显高于对照组（p〈0.05,p〈0.01）,高剂量组雌性大鼠脑、肝重量系数明显高于对照组（p〈0.05,p〈0.01）。病理组织学检查：高剂量组一只雌性大鼠肾小管管型;其它各脏器组织病理学检查未见明显病理改变。结论：除草啶原药对雄、雌性大鼠经口染毒剂量为215.0 mg/kg bw.d及以上时,对大鼠有毒性效应。除草啶原药对雄、雌性大鼠亚慢性经口毒性最大无作用剂量为107.5 mg/kg bw.d。除草啶原药对血脂代谢产生了一定影响。觀察除草啶經口飼餵90 d對大鼠血液、尿液、髒器重,生化指標及病理組織學的變化。依據GB15670-1995《農藥登記毒理學試驗方法》對其進行瞭為期90 d的毒性實驗。結果,試驗期間各組動物一般情況良好,無死亡、厭食和其它異常行為;中、高劑量組雄、雌性大鼠週平均體重從第2週開始至實驗結束均明顯低于對照組（p〈0.05,p〈0.01）;高劑量組雄、雌性大鼠的食物消耗量明顯低于對照組（p〈0.05）。血液學檢查可見中劑量組雄性大鼠WBC明顯低于對照組（p〈0.01）,但無劑量-反應關繫。中、高劑量組雄性大鼠RBC和HGB均明顯低于對照組（p〈0.01）;雌性大鼠的血常規檢查未見明顯改變。血清生化測定結果可見雄性大鼠中劑量組的BUN明顯低于對照組（p〈0.05）,但無劑量-反應關繫;中、高劑量組的TG明顯低于對照組（p〈0.05、p〈0.01）,高劑量組的GLU明顯低于對照組（p〈0.05）,提示受檢樣品對雄性大鼠糖代謝和脂代謝有一定影響;雌性大鼠中、高劑量組的ALP明顯低于對照組（p〈0.05,p〈0.01）,高劑量的AST明顯高于對照組（p〈0.05）、GLU明顯低于對照組（p〈0.05）,提示受檢樣品對雌性大鼠肝功能、糖代謝有一定影響。中劑量的CHE明顯高于對照組（p〈0.01）,但無劑量-反應關繫。尿常規檢查：各劑量組與對照組比較,各項檢測指標無明顯變化。髒器檢查和髒器重量繫數測定：試驗結束進行大體解剖,各劑量組動物大體解剖結果均未見異常。髒器重量繫數測定結果錶明,中、高劑量組雄性大鼠腦、肝、腎、睪汍重量繫數明顯高于對照組（p〈0.05,p〈0.01）,高劑量組雌性大鼠腦、肝重量繫數明顯高于對照組（p〈0.05,p〈0.01）。病理組織學檢查：高劑量組一隻雌性大鼠腎小管管型;其它各髒器組織病理學檢查未見明顯病理改變。結論：除草啶原藥對雄、雌性大鼠經口染毒劑量為215.0 mg/kg bw.d及以上時,對大鼠有毒性效應。除草啶原藥對雄、雌性大鼠亞慢性經口毒性最大無作用劑量為107.5 mg/kg bw.d。除草啶原藥對血脂代謝產生瞭一定影響。
관찰제초정경구사위90 d대대서혈액、뇨액、장기중,생화지표급병리조직학적변화。의거GB15670-1995《농약등기독이학시험방법》대기진행료위기90 d적독성실험。결과,시험기간각조동물일반정황량호,무사망、염식화기타이상행위;중、고제량조웅、자성대서주평균체중종제2주개시지실험결속균명현저우대조조（p〈0.05,p〈0.01）;고제량조웅、자성대서적식물소모량명현저우대조조（p〈0.05）。혈액학검사가견중제량조웅성대서WBC명현저우대조조（p〈0.01）,단무제량-반응관계。중、고제량조웅성대서RBC화HGB균명현저우대조조（p〈0.01）;자성대서적혈상규검사미견명현개변。혈청생화측정결과가견웅성대서중제량조적BUN명현저우대조조（p〈0.05）,단무제량-반응관계;중、고제량조적TG명현저우대조조（p〈0.05、p〈0.01）,고제량조적GLU명현저우대조조（p〈0.05）,제시수검양품대웅성대서당대사화지대사유일정영향;자성대서중、고제량조적ALP명현저우대조조（p〈0.05,p〈0.01）,고제량적AST명현고우대조조（p〈0.05）、GLU명현저우대조조（p〈0.05）,제시수검양품대자성대서간공능、당대사유일정영향。중제량적CHE명현고우대조조（p〈0.01）,단무제량-반응관계。뇨상규검사：각제량조여대조조비교,각항검측지표무명현변화。장기검사화장기중량계수측정：시험결속진행대체해부,각제량조동물대체해부결과균미견이상。장기중량계수측정결과표명,중、고제량조웅성대서뇌、간、신、고환중량계수명현고우대조조（p〈0.05,p〈0.01）,고제량조자성대서뇌、간중량계수명현고우대조조（p〈0.05,p〈0.01）。병리조직학검사：고제량조일지자성대서신소관관형;기타각장기조직병이학검사미견명현병리개변。결론：제초정원약대웅、자성대서경구염독제량위215.0 mg/kg bw.d급이상시,대대서유독성효응。제초정원약대웅、자성대서아만성경구독성최대무작용제량위107.5 mg/kg bw.d。제초정원약대혈지대사산생료일정영향。
To achieve the largest non-role of parameters for the safe production and chronic toxicity test to provide a reference dose,Subjects Bromacil were defined toxicity characteristics.We are based on GB15670-1995 ＂registered pesticide toxicology test methods＂of its 90 d toxicity test.Results：The average weeks weight of the high-dose group male rats from the second weeks to the end of the experiment was significantly lower than that of the control group（p0.05,p0.01）,the WBC of the middle and high dose group male rats was significantly lower than that of the control group（p0.05）,suggesting that subjects materials have some inhibitory effect on the WBC and the growth of male rats,the RBC and HGB of the high-dose group female rats was significantly lowerr than that of the control group（p0.01）,The PLT,TG and GLU of the middle-dose group male,female rats was significantly lower than that of the control group（p0.01）,prompted subjects materials have a certain influence on rat liver function and carbohydrate metabolism,organ weight results show that the kidney weight of the middle and high-dose group male rats and the high-dose group female rats was significantly higher than that of the control group（p0.01,p0.05）,the liver weight of the middle and high dose group male and female rats was significantly higher than that of the control group（p0.01,p0.05）,prompted the brain,the kidney,the liver and the spermary have been compensation function changes,the histopathological examination has not found organic changes and the dose group was no significant difference as compared with the control group.Conclusion：Bromacil male rats and female rats exposed to oral doses of 215 mg/kg bw.d and above,having the toxic effect on rats.Therefore,in this experiment,the largest no role dose of male and female was 107.50 mg/kg bw.d.Bromacil had an impacts on lipid and blood sugar metabolism.