集美大学学报:自然科学版
集美大學學報:自然科學版
집미대학학보:자연과학판
Journal of Jimei University(Natural Science)
2012年
4期
253-258
,共6页
王玉%关瑞章%黎中宝%林鹏%郭松林
王玉%關瑞章%黎中寶%林鵬%郭鬆林
왕옥%관서장%려중보%림붕%곽송림
鳗鲡%气单胞菌%生化鉴定%AFLP%DNA分型
鰻鱺%氣單胞菌%生化鑒定%AFLP%DNA分型
만려%기단포균%생화감정%AFLP%DNA분형
eels%Aeromonas sp.%biochemistry identification%AFLP%DNA fingerprint typing
以32株从养殖鳗鲡分离的病原性气单胞菌为试验材料,采用Biolog自动菌鉴系统对其进行生理生化鉴定,结果表明,有20株被鉴定为嗜水气单胞菌,7株被鉴定为威隆气单胞菌,1株为豚鼠气单胞菌,其余4株未能鉴定到种.为进一步确定20株嗜水气单胞菌和7株威隆气单胞菌的基因型,筛选了2对引物(E-C/M-C,E-C/M-A)分别对这两个菌种进行了AFLP分析.结果表明:20株嗜水气单胞菌中共检测到88个位点,均为多态性位点;7株威隆气单胞菌中共检测到61个位点,其中60个为多态性位点.经UPGMA聚类分析表明:20株嗜水气单胞菌可初步分为3种类型,每种类型之间平均遗传距离为0.449,同一类型内不同菌株的平均遗传距离为0.127;7株威隆气单胞菌可初步分为4种类型,每种类型之间平均遗传距离为0.467,变异范围为0.415~0.525.该结果为鱼类病原菌的DNA分型研究提供了参考.
以32株從養殖鰻鱺分離的病原性氣單胞菌為試驗材料,採用Biolog自動菌鑒繫統對其進行生理生化鑒定,結果錶明,有20株被鑒定為嗜水氣單胞菌,7株被鑒定為威隆氣單胞菌,1株為豚鼠氣單胞菌,其餘4株未能鑒定到種.為進一步確定20株嗜水氣單胞菌和7株威隆氣單胞菌的基因型,篩選瞭2對引物(E-C/M-C,E-C/M-A)分彆對這兩箇菌種進行瞭AFLP分析.結果錶明:20株嗜水氣單胞菌中共檢測到88箇位點,均為多態性位點;7株威隆氣單胞菌中共檢測到61箇位點,其中60箇為多態性位點.經UPGMA聚類分析錶明:20株嗜水氣單胞菌可初步分為3種類型,每種類型之間平均遺傳距離為0.449,同一類型內不同菌株的平均遺傳距離為0.127;7株威隆氣單胞菌可初步分為4種類型,每種類型之間平均遺傳距離為0.467,變異範圍為0.415~0.525.該結果為魚類病原菌的DNA分型研究提供瞭參攷.
이32주종양식만려분리적병원성기단포균위시험재료,채용Biolog자동균감계통대기진행생리생화감정,결과표명,유20주피감정위기수기단포균,7주피감정위위륭기단포균,1주위돈서기단포균,기여4주미능감정도충.위진일보학정20주기수기단포균화7주위륭기단포균적기인형,사선료2대인물(E-C/M-C,E-C/M-A)분별대저량개균충진행료AFLP분석.결과표명:20주기수기단포균중공검측도88개위점,균위다태성위점;7주위륭기단포균중공검측도61개위점,기중60개위다태성위점.경UPGMA취류분석표명:20주기수기단포균가초보분위3충류형,매충류형지간평균유전거리위0.449,동일류형내불동균주적평균유전거리위0.127;7주위륭기단포균가초보분위4충류형,매충류형지간평균유전거리위0.467,변이범위위0.415~0.525.해결과위어류병원균적DNA분형연구제공료삼고.
Thirty-two pathogens isolated from diseased eels and classified as the genus of Aromonas sp.were further identified by an automatic biochemical identification system of Biolog.The results showed that 20 pathogens were identified as Aromonas hyplrophila,7 pathogehs were Aromonas veronii,and 1 pathogen was Aromonas caviae,respectively.Another 4 strains were identified as the genus of Aromonas.The 20 strains of Aromonas hydrophila and 7 strains of Aromonas veronii were then analyzed by Amplified Fragment Length Polymorphism(AFLP) to ascertain the DNA finger typing.The results showed that 88 loci were amplified by two pairs of primers(E-C/M-C and E-C/M-A) with all polymorphic loci in Aromonas hydrophila and 61 loci were amplified by the same primers with 60 polymorphic loci in Aromonas veronii.The UPGMA(Unweighted Pair Group Method with Arithmetic Mean Analysis) dendrogram showed that 20 strains of Aromonas hydrophila and 7 strains of Aromonas veronii can be primarily divided into 3 and 4 DNA finger typing respectively.The average distance of gene diversity between and in 3 DNA finger typing of Aromonas hydrophila was 0.449 and 0.127 respectively.The average distance of gene diversity between 4 DNA finger typing of Aromonas veronii was 0.467 and ranged from 0.415 to 0.525.The research provided a new technology for the DNA finger typing of fish pathogens.