激光杂志
激光雜誌
격광잡지
LASER JOURNAL
2012年
4期
88-89
,共2页
刘典锋%周玥%蔡世忠%何轩%耿珊%张琛%孙可%王亚平
劉典鋒%週玥%蔡世忠%何軒%耿珊%張琛%孫可%王亞平
류전봉%주모%채세충%하헌%경산%장침%손가%왕아평
气虚血虚%动物模型%造血干/祖细胞%衰老
氣虛血虛%動物模型%造血榦/祖細胞%衰老
기허혈허%동물모형%조혈간/조세포%쇠로
deficiency qi- bloed%animal model%HSG/HPC%aging
目的：构建中医“气虚血虚”模型，探讨造血干／祖细胞（HSC／HPC）衰老及检测相关生物学指标，为阐释中医“气虚血虚”的现代生物学机理和探讨“补气生血”药物的作用提供理论与实验依据。方法：6．5Gy的X射线一次性全身辐射C57小鼠，辐射6h内腹腔注射盐酸苯肼40mg/kg，建立“气虚血虚”模型（即骨髓抑制与贫血模型），分别在第3、7、14、28d观察小鼠的行为学变化、外周血象、骨髓单个核细胞总数、免疫磁性分选法分离鉴定小鼠Sca-1＋HSC／HPC、检测骨髓单个核细胞数（MNCs）与造血干／祖细胞（Sca-1^＋HSC／HPC）数量、衰老SA-β-半乳糖苷酶染色检测Sca-1^＋HSC／HPC阳性率、Sca-1^＋HSC／HPC细胞周期分布、脾指数与脾脏病理变化。结果：与对照组相比，在各时间段上模型组小鼠均表现出精神萎靡，行为迟缓，进食量下降等中医“气虚血虚”症候、外周血象各项指标降低，MNCs数下降，Sca-1^＋HSC／HPC数下降、SA—β-半乳糖苷酶染色阳性百分率（11．82±3．24）％，高于对照组[（2．07±2．21）％，P〈0．05]、细胞周期G1期阻滞，G0／G1期比例为（86．70±3．79）％，高于对照组[（69．31±3．07）％，P〈0．05]，S期比例减少、脾指数降低和脾脏退行性变化显著。结论：辐射和盐酸苯肼并用法可成功的建立中医“气虚血虚”动物模型，并导致造血干／祖细胞衰老。
目的：構建中醫“氣虛血虛”模型，探討造血榦／祖細胞（HSC／HPC）衰老及檢測相關生物學指標，為闡釋中醫“氣虛血虛”的現代生物學機理和探討“補氣生血”藥物的作用提供理論與實驗依據。方法：6．5Gy的X射線一次性全身輻射C57小鼠，輻射6h內腹腔註射鹽痠苯肼40mg/kg，建立“氣虛血虛”模型（即骨髓抑製與貧血模型），分彆在第3、7、14、28d觀察小鼠的行為學變化、外週血象、骨髓單箇覈細胞總數、免疫磁性分選法分離鑒定小鼠Sca-1＋HSC／HPC、檢測骨髓單箇覈細胞數（MNCs）與造血榦／祖細胞（Sca-1^＋HSC／HPC）數量、衰老SA-β-半乳糖苷酶染色檢測Sca-1^＋HSC／HPC暘性率、Sca-1^＋HSC／HPC細胞週期分佈、脾指數與脾髒病理變化。結果：與對照組相比，在各時間段上模型組小鼠均錶現齣精神萎靡，行為遲緩，進食量下降等中醫“氣虛血虛”癥候、外週血象各項指標降低，MNCs數下降，Sca-1^＋HSC／HPC數下降、SA—β-半乳糖苷酶染色暘性百分率（11．82±3．24）％，高于對照組[（2．07±2．21）％，P〈0．05]、細胞週期G1期阻滯，G0／G1期比例為（86．70±3．79）％，高于對照組[（69．31±3．07）％，P〈0．05]，S期比例減少、脾指數降低和脾髒退行性變化顯著。結論：輻射和鹽痠苯肼併用法可成功的建立中醫“氣虛血虛”動物模型，併導緻造血榦／祖細胞衰老。
목적：구건중의“기허혈허”모형，탐토조혈간／조세포（HSC／HPC）쇠로급검측상관생물학지표，위천석중의“기허혈허”적현대생물학궤리화탐토“보기생혈”약물적작용제공이론여실험의거。방법：6．5Gy적X사선일차성전신복사C57소서，복사6h내복강주사염산분정40mg/kg，건립“기허혈허”모형（즉골수억제여빈혈모형），분별재제3、7、14、28d관찰소서적행위학변화、외주혈상、골수단개핵세포총수、면역자성분선법분리감정소서Sca-1＋HSC／HPC、검측골수단개핵세포수（MNCs）여조혈간／조세포（Sca-1^＋HSC／HPC）수량、쇠로SA-β-반유당감매염색검측Sca-1^＋HSC／HPC양성솔、Sca-1^＋HSC／HPC세포주기분포、비지수여비장병리변화。결과：여대조조상비，재각시간단상모형조소서균표현출정신위미，행위지완，진식량하강등중의“기허혈허”증후、외주혈상각항지표강저，MNCs수하강，Sca-1^＋HSC／HPC수하강、SA—β-반유당감매염색양성백분솔（11．82±3．24）％，고우대조조[（2．07±2．21）％，P〈0．05]、세포주기G1기조체，G0／G1기비례위（86．70±3．79）％，고우대조조[（69．31±3．07）％，P〈0．05]，S기비례감소、비지수강저화비장퇴행성변화현저。결론：복사화염산분정병용법가성공적건립중의“기허혈허”동물모형，병도치조혈간／조세포쇠로。
Objective： To establish the model of deficiency qi - blood and investigate the aging Sca- 1^＋ HSC/HPC relevant biological indicators. The purpose is to explain the mechanism of deficiency qi - blood and build the foundation of studying the mechanism of qi - blood- tonic. Methods：Mice （ C57BL/6} were dealt with 6.5Gy x - ray for one - time total body irradiaticn （ TBI }, within 6h , lntraperitoneal injection of phonylhydrasine hydreehloride for 40mg/kg. establishing the model of bone marrow suppression and hemolytic anemia, watch the behavior of mice and assay the number of MNCs and Sca- 1^＋ HSC/HPC, senescence- associated β- galactasidase {SA- β- gal）cytochemical staining, The ratio of aging Sca- 1^＋ HSC/HPC entered G1 phase of the cell cycle, spleen index and biopsy. Results：Compared with the control group, apathetic expression, behavioral retardatien,decreased food intake were observed, the routine bloed test were dramatically declined, the number of MNCs and Sca- 1^＋ HSC/HPC were significantly reduced, the positive staining rate and the ratio of Sca - 1^＋ HSC/HPC were higher in model group than in control group （11.82% ± 3.24% vs 2.07%±2.21% ,86.70%±3.79% vs 69.31%±3.07%, P 〈 0.05）, the spleen index was significantly reduced and the spleen had Significant degenerative changes. Conclusion： The way of usingx- ray and phenylhydrazine hydrochloride can successfully establish the model of deficiency qi - blood and induce Sca- 1 ^＋ HSC/HPC aging.
