激光杂志
激光雜誌
격광잡지
LASER JOURNAL
2012年
4期
76-77
,共2页
陈倩%陈雪梅%何俊琳%王应雄%丁裕斌%杨德辉%刘学庆
陳倩%陳雪梅%何俊琳%王應雄%丁裕斌%楊德輝%劉學慶
진천%진설매%하준림%왕응웅%정유빈%양덕휘%류학경
microRNAs%hsa-miR-200a%真核表达载体%肿瘤
microRNAs%hsa-miR-200a%真覈錶達載體%腫瘤
microRNAs%hsa-miR-200a%진핵표체재체%종류
microRNAs%hsa- miR- 200a%eukaryotic expression yector%tumor
目的：通过构建及鉴定miR-200a的真核表达载体pcDNA3．1（＋）-miR-200a，为进一步研究miR-200a的功能奠定实验基础。方法：RT-PCR扩增pre—miR-200a基因序列，连接于表达载体pcDNA3．1（＋）中，对重组质粒双酶切鉴定并测序验证。结果：pcDNA3．1（＋）-miR-200a真核表达载体经酶切及测序鉴定正确。结论：pcDNA3．1（＋）-miR-200a真核表达载体构建成功，为下一步深入研究miR-200a的生物学功能和验证miR-200a的靶基因提供有效工具。
目的：通過構建及鑒定miR-200a的真覈錶達載體pcDNA3．1（＋）-miR-200a，為進一步研究miR-200a的功能奠定實驗基礎。方法：RT-PCR擴增pre—miR-200a基因序列，連接于錶達載體pcDNA3．1（＋）中，對重組質粒雙酶切鑒定併測序驗證。結果：pcDNA3．1（＋）-miR-200a真覈錶達載體經酶切及測序鑒定正確。結論：pcDNA3．1（＋）-miR-200a真覈錶達載體構建成功，為下一步深入研究miR-200a的生物學功能和驗證miR-200a的靶基因提供有效工具。
목적：통과구건급감정miR-200a적진핵표체재체pcDNA3．1（＋）-miR-200a，위진일보연구miR-200a적공능전정실험기출。방법：RT-PCR확증pre—miR-200a기인서렬，련접우표체재체pcDNA3．1（＋）중，대중조질립쌍매절감정병측서험증。결과：pcDNA3．1（＋）-miR-200a진핵표체재체경매절급측서감정정학。결론：pcDNA3．1（＋）-miR-200a진핵표체재체구건성공，위하일보심입연구miR-200a적생물학공능화험증miR-200a적파기인제공유효공구。
Objective： To construct and identify the eukaryotic expressiou vector pcDNA3.1 （ ＋ ） - miR - 200a of hsa - miR - 200a. It may lay a foundation for further studying the function of hsa - miR - 200a. Methods： Amplified by reverse transcription - polymerase chain reaction（ RT - PCR）, The pre - miR - 200a sequence was inserted into pcDNA3.1 （ ＋ ） vector. The recombinant plasmid of pcDNA3.1 （ ＋ ） - miR - 200a was confirmed by restriction endnuclease analysis as well as DNA sequencing. Results： The result of DNA sequencing demonstrated that eukaryotic vector pcDNA3.1 （ ＋ ） - miR- 200a was completely correct. Conclusion： The successful construction of eukaryotic expression vector of hsa - miR - 200a provided a useful tool for the study of biological function of hsa - miR - 200a and its potential target genes.
