CAJ | 학술논문

目的：研究双氢青蒿素（Dihydroatemisinine，DHA）对人骨肉瘤细胞143B的影响及所导致的形态学变化。方法：采用人骨肉瘤143B细胞株，通过设置对照组和低浓度、中浓度、高浓度的DHA组，H．E染色观察Trandwell小室穿梭能力；划痕愈合实验观察迁移能力；Hoechst33258荧光染色检测细胞凋亡；MTT法检测细胞增殖抑制能力。结果：DHA表现出明显地抑制人骨肉瘤143B细胞的迁移、增殖以及促进凋亡（P〈0．05），且伴随浓度的递增和时间的延长，抑制作用更明显。结论：DHA具有较强的抗入骨肉瘤作用，其机制可能与DHA诱导骨肉瘤细胞凋亡，并抑制其增殖有关。
목적：연구쌍경청호소（Dihydroatemisinine，DHA）대인골육류세포143B적영향급소도치적형태학변화。방법：채용인골육류143B세포주，통과설치대조조화저농도、중농도、고농도적DHA조，H．E염색관찰Trandwell소실천사능력；화흔유합실험관찰천이능력；Hoechst33258형광염색검측세포조망；MTT법검측세포증식억제능력。결과：DHA표현출명현지억제인골육류143B세포적천이、증식이급촉진조망（P〈0．05），차반수농도적체증화시간적연장，억제작용경명현。결론：DHA구유교강적항입골육류작용，기궤제가능여DHA유도골육류세포조망，병억제기증식유관。
Objective： To study dihydroartemisinin in human esteosarcoma cells 143B and the resulting impact of morphological changes. Method：using human osteosarcoma 143B cell line, by setting the control group, low cencentrations, concentration, high concentrations DHA group, H. E staining Trandwell chamber shuttle capacity; experimental observation of scratch healing migration; Hcechst 33258 staining to detect apoptesis; MTT cell proliferation assay suppression. The results showed significantly inhibit DHA in human osteosarcema 143B cell migration, proliferation and promote apoptesis （ P 〈 0.05 ）, and with increasing concentration and time extension, the trend is more obvious. Conclusion： DHA has strong anti - osteosarcoma effects, its mechanism may be related to induction of apoptosis in osteosarcoma cells, and inhibition of proliferation.