激光杂志
激光雜誌
격광잡지
LASER JOURNAL
2012年
4期
58-60
,共3页
蒋金%曹友德%磨娜%李静%莫显刚
蔣金%曹友德%磨娜%李靜%莫顯剛
장금%조우덕%마나%리정%막현강
姜黄素%细胞凋亡%P53
薑黃素%細胞凋亡%P53
강황소%세포조망%P53
curcumin%Cell Apoptosis%P53.
目的：研究姜黄素对A549细胞凋亡的诱导作用，并探讨其可能的分子机制。方法：姜黄素处理A549细胞后，MTT法于不同时间点检测A549细胞的增殖情况；姜黄素处理A549细胞48h后，流式细胞术（Flow CytoMetry，FCM）检测A549细胞的凋亡率；30μmol／L姜黄素处理A549细胞24h、48h、72h后，RT—PCR、Western—Blot检测A549细胞中P53、Bcl2、Bax和cagpase-3基因的表达水平。结果：姜黄素对肺癌A549细胞增殖的抑制作用具有显著的浓度-时间依赖关系，30μmol／L姜黄素可诱导A549细胞凋亡。30μmoL／L姜黄素作用A549细胞24h即可引起A549细胞中P53、Bax和Caspase-3表达水平的上调，48h后可引起Bcl2表达水平的下调，诱导A549细胞凋亡。结论：30μmol／L姜黄素可诱导A549细胞凋亡，其机制可能与促进P53、Bax和Caspase-3基因的表达，抑制Bcl2基因的表达有关。
目的：研究薑黃素對A549細胞凋亡的誘導作用，併探討其可能的分子機製。方法：薑黃素處理A549細胞後，MTT法于不同時間點檢測A549細胞的增殖情況；薑黃素處理A549細胞48h後，流式細胞術（Flow CytoMetry，FCM）檢測A549細胞的凋亡率；30μmol／L薑黃素處理A549細胞24h、48h、72h後，RT—PCR、Western—Blot檢測A549細胞中P53、Bcl2、Bax和cagpase-3基因的錶達水平。結果：薑黃素對肺癌A549細胞增殖的抑製作用具有顯著的濃度-時間依賴關繫，30μmol／L薑黃素可誘導A549細胞凋亡。30μmoL／L薑黃素作用A549細胞24h即可引起A549細胞中P53、Bax和Caspase-3錶達水平的上調，48h後可引起Bcl2錶達水平的下調，誘導A549細胞凋亡。結論：30μmol／L薑黃素可誘導A549細胞凋亡，其機製可能與促進P53、Bax和Caspase-3基因的錶達，抑製Bcl2基因的錶達有關。
목적：연구강황소대A549세포조망적유도작용，병탐토기가능적분자궤제。방법：강황소처리A549세포후，MTT법우불동시간점검측A549세포적증식정황；강황소처리A549세포48h후，류식세포술（Flow CytoMetry，FCM）검측A549세포적조망솔；30μmol／L강황소처리A549세포24h、48h、72h후，RT—PCR、Western—Blot검측A549세포중P53、Bcl2、Bax화cagpase-3기인적표체수평。결과：강황소대폐암A549세포증식적억제작용구유현저적농도-시간의뢰관계，30μmol／L강황소가유도A549세포조망。30μmoL／L강황소작용A549세포24h즉가인기A549세포중P53、Bax화Caspase-3표체수평적상조，48h후가인기Bcl2표체수평적하조，유도A549세포조망。결론：30μmol／L강황소가유도A549세포조망，기궤제가능여촉진P53、Bax화Caspase-3기인적표체，억제Bcl2기인적표체유관。
Objective：To study the induced effect of curcumin on apoptosis in Lung Cancer 3-549 Cells and explore the possible mechanisms. Methods： The proliferation of A549 cells was detected by MTT assay at different time points after curcumin treatment; the apoptosis of A549 cells was detected by FCM at 48 hours after curcumin treatment. The expression levels of P53, Bcl2, Bax, Caspase - 3 were detected by RT - PCR, Western - Blot at 48 hours after curcumin treatment. Result： Inhibitory effect of curcumin on proliferation in Lung Cancer 3.549 Cells has a significant concentration- time dependent manner. 30μmol/L can induce the apoptosis of A549 cells. The expression levels of P53, Bax and Caspase - 3 on A549 cells were up - regulation at 24 hours after 30μmol / L curcumin treatment; the expression level of Bcl2 on A549 cells was down - regulation at 48 hours after 30μmol/L curcumin treatment; the apoptosis of A549 cells was induced at 48 hours after 30μmol/L curcumin treatment. Conclusion：30μmol/L curcumin can induce the apoptosis of A549 cells, the mechanism may be related to the promotion of P53, Bax and Caspase - 3 gene expression, inhibition of Bcl2 gene expression.
