激光杂志
激光雜誌
격광잡지
LASER JOURNAL
2012年
4期
46-47
,共2页
曾佳%高志%邱丽华%李倩%伍明毅
曾佳%高誌%邱麗華%李倩%伍明毅
증가%고지%구려화%리천%오명의
人舌鳞癌Tca8113细胞%竹红菌乙素(HB)%光动力治疗(PDT)%ROS
人舌鱗癌Tca8113細胞%竹紅菌乙素(HB)%光動力治療(PDT)%ROS
인설린암Tca8113세포%죽홍균을소(HB)%광동력치료(PDT)%ROS
human tongue carcinoma Tca8113 cell%hypocrellinB ( HB )%photedynamic therapy ( PDT )%ROS
目的：本文是用实验的方法，初步探讨中药光敏剂竹红菌乙素（HB）在光动力（PDT）作用下，对体外人舌鳞癌Tca8113细胞的杀伤作用。方法：常规培养、选取对数生长期人舌鳞癌Tca8113细胞，用含有竹红菌乙素（HB）浓度分别为0μM、0．25μM、0．5μ／M、1μM、2μM的RPMI-1640培养液孵育细胞4h后，经470nmLED定制多光源半导体激光光照处理（PDT），能量密度设置分别为0J／CM^2、1J／CM^2、2J／CM^2、3J／CM^2、4J／CM^2。PDT处理后细胞继续孵育24h后，分别在光学显微镜和荧光显微镜下观察细胞形态学变化，并用甲基噻唑四唑法（MIT法）检测竹红菌乙素（HB）对细胞的抑制作用。并分别在激光照射6h及24h后，用流式细胞仪测定细胞凋亡率。结果：经PDT处理后细胞在光学显微镜及荧光显微镜下均可观察到细胞坏死和凋亡样改变；在一定浓度范围和光照能量密度范围内，竹红菌乙素（HB）在PDT作用下对人舌鳞癌Tca8113细胞生长增殖的抑制和诱导凋亡作用与药物浓度和能量密度成正相关变化。结论：竹红菌乙素（HB）在光动力（PDT）作用下，对人舌鳞癌Tca8113细胞具有显著的杀伤作用，并在一定范围内呈浓度剂量和光剂量正相关依赖性。
目的：本文是用實驗的方法，初步探討中藥光敏劑竹紅菌乙素（HB）在光動力（PDT）作用下，對體外人舌鱗癌Tca8113細胞的殺傷作用。方法：常規培養、選取對數生長期人舌鱗癌Tca8113細胞，用含有竹紅菌乙素（HB）濃度分彆為0μM、0．25μM、0．5μ／M、1μM、2μM的RPMI-1640培養液孵育細胞4h後，經470nmLED定製多光源半導體激光光照處理（PDT），能量密度設置分彆為0J／CM^2、1J／CM^2、2J／CM^2、3J／CM^2、4J／CM^2。PDT處理後細胞繼續孵育24h後，分彆在光學顯微鏡和熒光顯微鏡下觀察細胞形態學變化，併用甲基噻唑四唑法（MIT法）檢測竹紅菌乙素（HB）對細胞的抑製作用。併分彆在激光照射6h及24h後，用流式細胞儀測定細胞凋亡率。結果：經PDT處理後細胞在光學顯微鏡及熒光顯微鏡下均可觀察到細胞壞死和凋亡樣改變；在一定濃度範圍和光照能量密度範圍內，竹紅菌乙素（HB）在PDT作用下對人舌鱗癌Tca8113細胞生長增殖的抑製和誘導凋亡作用與藥物濃度和能量密度成正相關變化。結論：竹紅菌乙素（HB）在光動力（PDT）作用下，對人舌鱗癌Tca8113細胞具有顯著的殺傷作用，併在一定範圍內呈濃度劑量和光劑量正相關依賴性。
목적：본문시용실험적방법，초보탐토중약광민제죽홍균을소（HB）재광동력（PDT）작용하，대체외인설린암Tca8113세포적살상작용。방법：상규배양、선취대수생장기인설린암Tca8113세포，용함유죽홍균을소（HB）농도분별위0μM、0．25μM、0．5μ／M、1μM、2μM적RPMI-1640배양액부육세포4h후，경470nmLED정제다광원반도체격광광조처리（PDT），능량밀도설치분별위0J／CM^2、1J／CM^2、2J／CM^2、3J／CM^2、4J／CM^2。PDT처리후세포계속부육24h후，분별재광학현미경화형광현미경하관찰세포형태학변화，병용갑기새서사서법（MIT법）검측죽홍균을소（HB）대세포적억제작용。병분별재격광조사6h급24h후，용류식세포의측정세포조망솔。결과：경PDT처리후세포재광학현미경급형광현미경하균가관찰도세포배사화조망양개변；재일정농도범위화광조능량밀도범위내，죽홍균을소（HB）재PDT작용하대인설린암Tca8113세포생장증식적억제화유도조망작용여약물농도화능량밀도성정상관변화。결론：죽홍균을소（HB）재광동력（PDT）작용하，대인설린암Tca8113세포구유현저적살상작용，병재일정범위내정농도제량화광제량정상관의뢰성。
Objective： To primarily explore the lethal effects of traditional Chinese drag photosensitizer hypoerellinB with photedynamic therapy （PDT） on human tongue carcinoma Tca8113 cells in vitro. Methods： Logarithmic growing Tca8113 coils were incubated in RPMI - 1640 medium with hypocrellinB for 5 different concentrations （0μM, 0.25gM, 0.5μM,1 μM, 2μM） for 4 hours, then the chosen cells were irradiated with PI）T by 470nm LED multi - lighthouse semiconductor laser. The light enegy density was decemed to 5 ladders （0J/CM^2. 1 J/CM^2, 2 J/CM^2, 3J/CM^2. 4J/CM2 ）. After 24 hours later, the modality of the hypocrellinB- PDT cells was observed by light microscope and fluorescence microscope, and the proliferative inhibition of the Tca8113 cells was detected by methyl thiazelyhetrazol（MTT）. The coils apoptosis rate was determined by flow cytometry after 6 hours and 24 hotws . Results： The necrosis and apoptosis cells were both seen by light microscope and fluoresconco microscope in the PDT cells. Under definite coucontration range and energy density range, the inhibition rate and the induced apoptosis rate of the hypocrellinB- PDT cells were direct ratio correlative changed with the concentration and the energy density. Conclusion： HypocrellinB - PDT has conspicuous lethal effects on human tongue carcinoma Tea8113 cells, which has direct correlation with the concontration range and energy density range under definite extent.
