CAJ | 학술논문

羊口疮（Orf）是由羊口疮病毒（ORFV）引起的人畜共患的一种急性、接触性和具有高度嗜上皮性传染病,主要感染绵羊、山羊和人。由于该病缺乏全身性感染症状,因此在防治上也缺乏有效的疫苗或抗体。RNA干扰技术是目前较为成熟的抑制目的基因表达生物技术。ORFV的DNA ploymerase是病毒复制的关键酶。研究运用RNA干扰技术针对ORFV的DNAploymerase基因进行体外基因沉默研究,通过网络自动筛选平台设计并合成3个short-hairpin RNAs（shRNAs）片段,并与含U6启动子的pLL3.7质粒构建重组载体,结果表明pLL3.7-D596为重组阳性,进一步测序结果正确。研究可以为靶向ORFV-DNAploymerase基因的体内基因沉默提供参考数据。
양구창（Orf）시유양구창병독（ORFV）인기적인축공환적일충급성、접촉성화구유고도기상피성전염병,주요감염면양、산양화인。유우해병결핍전신성감염증상,인차재방치상야결핍유효적역묘혹항체。RNA간우기술시목전교위성숙적억제목적기인표체생물기술。ORFV적DNA ploymerase시병독복제적관건매。연구운용RNA간우기술침대ORFV적DNAploymerase기인진행체외기인침묵연구,통과망락자동사선평태설계병합성3개short-hairpin RNAs（shRNAs）편단,병여함U6계동자적pLL3.7질립구건중조재체,결과표명pLL3.7-D596위중조양성,진일보측서결과정학。연구가이위파향ORFV-DNAploymerase기인적체내기인침묵제공삼고수거。
Orf（contagious ecthyma,contagious pustular dermatitis,scabby mouth） was an acute skin zoonosis caused by orf virus（ORFV）,which could infect sheep,goats and humans.Because of the lack of systemic infection symptom,the prevention was also short of effective vaccines or antibodies.RNA interference technology was the relatively mature biotechnology which was used to inhibit the expression of target genes.DNA polymerase of ORFV played an important role as key enzyme related to replication.In this study,RNA interference technology was applied to vitro gene silencing research on DNA polymerase gene of ORFV,by automatic screening platform of network,synthesized 3 shRNAs fragments which with pLL3.7 plasmid contained U6 promoter to build recombination vector.The result showed that pLL3.7-D596 was positive clone followed by sequencing,which could provide reference data to vivo gene silencing of DNA polymerase of ORFV.