贵州畜牧兽医
貴州畜牧獸醫
귀주축목수의
GUIZHOU ANIMAL SCIENCE AND VETERINARY MEDICINE
2012年
4期
1-3
,共3页
鲜猪肉%葡萄球菌%大肠杆菌%PCR
鮮豬肉%葡萄毬菌%大腸桿菌%PCR
선저육%포도구균%대장간균%PCR
Fresh Pork%Staphylococcus%E. coli%PCR
为了解市售鲜猪肉葡萄球菌和大肠杆菌污染情况，本试验从贵州省9个地区农贸市场采集鲜猪肉样本106份，3个地区屠宰场运输车辆采集棉拭子样本36份，采用传统生化法鉴定2种优势菌落；PCR法快速检测葡萄球菌和大肠杆菌。结果显示，鲜猪肉和棉拭子样本葡萄球菌的分离率为23．5％（38／162）和31．9％（23／72），大肠杆菌的分离率为15．4％（25／162）和13．9％（10／72）；2种细菌可扩增出目的条带。结果表明，市售鲜猪肉及运输车辆葡萄球菌和大肠杆菌污染严重，本试验成功运用PCR法对这2种病原菌进行快速检测。
為瞭解市售鮮豬肉葡萄毬菌和大腸桿菌汙染情況，本試驗從貴州省9箇地區農貿市場採集鮮豬肉樣本106份，3箇地區屠宰場運輸車輛採集棉拭子樣本36份，採用傳統生化法鑒定2種優勢菌落；PCR法快速檢測葡萄毬菌和大腸桿菌。結果顯示，鮮豬肉和棉拭子樣本葡萄毬菌的分離率為23．5％（38／162）和31．9％（23／72），大腸桿菌的分離率為15．4％（25／162）和13．9％（10／72）；2種細菌可擴增齣目的條帶。結果錶明，市售鮮豬肉及運輸車輛葡萄毬菌和大腸桿菌汙染嚴重，本試驗成功運用PCR法對這2種病原菌進行快速檢測。
위료해시수선저육포도구균화대장간균오염정황，본시험종귀주성9개지구농무시장채집선저육양본106빈，3개지구도재장운수차량채집면식자양본36빈，채용전통생화법감정2충우세균락；PCR법쾌속검측포도구균화대장간균。결과현시，선저육화면식자양본포도구균적분리솔위23．5％（38／162）화31．9％（23／72），대장간균적분리솔위15．4％（25／162）화13．9％（10／72）；2충세균가확증출목적조대。결과표명，시수선저육급운수차량포도구균화대장간균오염엄중，본시험성공운용PCR법대저2충병원균진행쾌속검측。
To investigate the contamination by Staphylococcus and E. coli of the saled fresh porks, 106 fresh pork samples were randomly collected from 9 farmer＇s markets, 36 cotton swab samples were randomly collected from the vehicles of 3 slaughterhouses for bacteriological isolation and identifica- tion. PCR detection for E. coli and Staphylococcus. The result showed that we isolated 38 strains Staphylococcus and 23 strains E. coli, the specific DNA fragments were detected by PCR. These indicated that there was a certain level of bacterial pollution in fresh pork, PCR methods for detecting E. coli and Staphylococcus were successfully established.
