CAJ | 학술논문

采用胶体金免疫层析技术（GICA）测定水产品中的呋喃妥因代谢物残留,并用液相色谱-串联质谱（LC-MS/MS）对实验结果进行确证。结果显示,胶体金免疫层析法的检测限为1.0μg/kg,假阳性率不大于5%,假阴性率为0;对实际样本的检测结果与LC-MS/MS法一致。研究表明,胶体金试纸条法操作简便、快速,成本低,为水产品中呋喃妥因代谢物的残留检测提供了快速筛选方法,而LC-MS/MS法灵敏度高,结果准确,适用于阳性样本的确证和精确定量。
채용효체금면역층석기술（GICA）측정수산품중적부남타인대사물잔류,병용액상색보-천련질보（LC-MS/MS）대실험결과진행학증。결과현시,효체금면역층석법적검측한위1.0μg/kg,가양성솔불대우5%,가음성솔위0;대실제양본적검측결과여LC-MS/MS법일치。연구표명,효체금시지조법조작간편、쾌속,성본저,위수산품중부남타인대사물적잔류검측제공료쾌속사선방법,이LC-MS/MS법령민도고,결과준학,괄용우양성양본적학증화정학정량。
Gold immunochromatographic assay （GICA） was used to determine the nitrofurantoin metabolite （AHD） residues in aquatic products, and also the liquid chromatographytandem mass spectrometry （LC - MS/MS） was used to validate the results. The detection limit of GICA was 1.0 μg/kg. The false positive rate was less 5 % , and false negative rate was 0. The results of the two methods for aquatic products were consistent. The results showed that GICA had the advantages of convenient operation, provided a rapid method for the screen of AHD in aquatic products, while LC - able for determination of positive samples. rapidity and low cost, which MS/MS was accurate and suit able for determination of positive samples.