福建水产
福建水產
복건수산
JOURNAL OF FUJIAN FISHERIES
2012年
4期
278-283
,共6页
杨章武%翁忠钗%姚志贤%卢小宁%张哲%陈政强
楊章武%翁忠釵%姚誌賢%盧小寧%張哲%陳政彊
양장무%옹충차%요지현%로소저%장철%진정강
凡纳滨对虾%群体选育%子代%免疫
凡納濱對蝦%群體選育%子代%免疫
범납빈대하%군체선육%자대%면역
Litopenaeus vannamei%selection breeding%offspring%immunology
在凡纳滨对虾群体选择育种研究中,利用1个经过人工选择和自交传代的凡纳滨对虾群体F2A,连续两个世代对该群体自交繁育的子代及其与其它群体杂交繁育的子代,进行免疫指标的检测和分析。结果表明,该群体两个世代的自交组繁育的子代F3A和F4A组,多项免疫指标都高于同世代的其它交配组,其中血细胞吞噬杀伤活性F3A组比同世代其它组高14.8%和64.3%,F4A组比同世代的其他组高27.0%~44.8%;血清溶细胞活性F3A组比同世代其它组高76.3%和74.6%,F4A组比同世代其它组高36.5%~58.3%;血清蛋白含量也高于其它组;只有酚氧化酶低于其它组。显示在人工选育条件下,凡纳滨对虾群体内自交传代,可能有利于群体免疫性能的遗传。但杂交组在生长和抗逆方面表现的杂交优势,与其偏低的免疫指标不一致。
在凡納濱對蝦群體選擇育種研究中,利用1箇經過人工選擇和自交傳代的凡納濱對蝦群體F2A,連續兩箇世代對該群體自交繁育的子代及其與其它群體雜交繁育的子代,進行免疫指標的檢測和分析。結果錶明,該群體兩箇世代的自交組繁育的子代F3A和F4A組,多項免疫指標都高于同世代的其它交配組,其中血細胞吞噬殺傷活性F3A組比同世代其它組高14.8%和64.3%,F4A組比同世代的其他組高27.0%~44.8%;血清溶細胞活性F3A組比同世代其它組高76.3%和74.6%,F4A組比同世代其它組高36.5%~58.3%;血清蛋白含量也高于其它組;隻有酚氧化酶低于其它組。顯示在人工選育條件下,凡納濱對蝦群體內自交傳代,可能有利于群體免疫性能的遺傳。但雜交組在生長和抗逆方麵錶現的雜交優勢,與其偏低的免疫指標不一緻。
재범납빈대하군체선택육충연구중,이용1개경과인공선택화자교전대적범납빈대하군체F2A,련속량개세대대해군체자교번육적자대급기여기타군체잡교번육적자대,진행면역지표적검측화분석。결과표명,해군체량개세대적자교조번육적자대F3A화F4A조,다항면역지표도고우동세대적기타교배조,기중혈세포탄서살상활성F3A조비동세대기타조고14.8%화64.3%,F4A조비동세대적기타조고27.0%~44.8%;혈청용세포활성F3A조비동세대기타조고76.3%화74.6%,F4A조비동세대기타조고36.5%~58.3%;혈청단백함량야고우기타조;지유분양화매저우기타조。현시재인공선육조건하,범납빈대하군체내자교전대,가능유리우군체면역성능적유전。단잡교조재생장화항역방면표현적잡교우세,여기편저적면역지표불일치。
The immune indices of detection and analysis on inbred and hybrid offspring within stock F2A through artificial selection and inbred during two successive generations were carried out in group selection breeding study of Litopenaeus vannaraei. The results showed that many immune indices in group F3A and F4A of inbred offspring were higher than the other mating groups in the same generation, including the phagocytic activity in the hemocytes for group F3A and F4A was 14. 8% to 64.3% and 27.0% to 44. 8% higher than the other groups, respectively. The cytolytic activity in the serotonin for group F3A and F4A was 76.3% to 74. 6% and 36.5% to 58.3% , higher than the other groups with the same generation. Serum protein levels were also higher than other groups, but only the index of prophenol oxidase was lower than other groups. The results indicated that inbred of L. vannamei may be benefit for heredity in immune. But heterosis of hybrid group in the growth rate and resistance was not consistent with its low immune indices.