CAJ | 학술논문

为了研究补充维生素c（Vc）对冷应激环境下雄性小鼠血清生化指标和部分内脏器官的影响。本试验选购清洁级雄性昆明小鼠30只，随机分为冷应激组、冷应激＋V。组和对照组（n=10），冷应激和冷应激＋Vc组每天在0℃下给2h的冷刺激，冷应激＋Vc组补充300μg／gVc，试验期1w。结果表明，在冷应激环境中，小鼠血清AST、UREA、UA、Glu、TP和CREA水平均出现显著变化（P〈0．05）；肾器官指数显著增加（P〈0．05），睾丸和附睾重量显著降低（P〈0．05）；肾、睾丸和附睾出现不同程度损伤，但肝的重量、器官指数和组织结构以及无明显变化。补充Vc后，血清uA明显降低（P〈0．05），G1u、1P和CREA含量显著增加（P〈0．05），而AsT和UREA含量无明显变化（P〉0．05）；肾器官指数显著降低（P〈0．05），睾丸重量明显增加（P〈0．05）；肾小球和肾小囊结构正常，睾丸生精细胞和精子数量增加，附睾管脱落上皮细胞数量减少。
위료연구보충유생소c（Vc）대랭응격배경하웅성소서혈청생화지표화부분내장기관적영향。본시험선구청길급웅성곤명소서30지，수궤분위랭응격조、랭응격＋V。조화대조조（n=10），랭응격화랭응격＋Vc조매천재0℃하급2h적랭자격，랭응격＋Vc조보충300μg／gVc，시험기1w。결과표명，재랭응격배경중，소서혈청AST、UREA、UA、Glu、TP화CREA수평균출현현저변화（P〈0．05）；신기관지수현저증가（P〈0．05），고환화부고중량현저강저（P〈0．05）；신、고환화부고출현불동정도손상，단간적중량、기관지수화조직결구이급무명현변화。보충Vc후，혈청uA명현강저（P〈0．05），G1u、1P화CREA함량현저증가（P〈0．05），이AsT화UREA함량무명현변화（P〉0．05）；신기관지수현저강저（P〈0．05），고환중량명현증가（P〈0．05）；신소구화신소낭결구정상，고환생정세포화정자수량증가，부고관탈락상피세포수량감소。
In order to research the effect of supplement vitamin C （ Vc ） on serum biochemical indicators and par- tial internal organ of male mice in cold stress environment, 30 clean level Kunming male mice were randomly di- vided into cold stress group, cold stress ＋ Vc group and control group （n = 10）. The cold stress and cold stress ＋ Vc group were given 2h cold stimulation at 0~C, and the cold stress ＋ Vc group were also given Vc of 300μg/ g BW, the experiment lasted for a week. The results showed that in cold stress environment, the level of serum AST, UREA, UA, Glu, TP and CREA changed significantly （P 〈0.05） ; the organ index of kidney had a sig- nificant increase （P 〈 0.05）, the weight of testis and epididymis were significantly reduced （ P 〈 0.05 ） ; the kidney, testis and damaged in epididymal different degree, but the weight, organ index and organizational struc- ture of liver were not obviously reduc （P 〉 0.05 ）. After complement Vc, the level of serum UA was significantly reduced （ P 〈 0.05 ）, and the level of serum Glu, TP and CREA were significantly increased （ P 〈 0.05 ）, but the level of serum AST and UREA had not significant changed （ P 〉 0.05 ） ; the organ index of kidney had a sig- nificant decrease （P 〈 0.05 ）, the weight of testis was obviously increased （ P 〈 0.05 ） ; the structure of glomeru- lar and renal capsule returned to normal, the spermatogenic cells and sperm were significantly increased, and thedeciduous epithelial cells in the epididymal ducts were decreased.