CAJ | 학술논문

目的：观察大蒜素对人肺腺癌细胞SLC-89增殖的影响。方法：不同浓度的大蒜素与SLC-89细胞共培养72 h,运用MTT法测定大蒜素的IC50。将IC50、IC50/2、IC50/4剂量的大蒜素与SLC-89细胞共培养72 h后,运用端粒重复序列扩增-酶联免疫吸附法分别测定细胞端粒酶活性,运用流式细胞技术测定细胞PCNA的表达率。加入等量生理盐水共培养作为对照组。结果：大蒜素能抑制SLC-89细胞的增殖,其IC50为79.8μg/mL,IC50和IC50/2剂量的大蒜素能显著抑制SLC-89细胞端粒酶的活性,IC50、IC50/2、IC50/4剂量的大蒜素都能显著抑制SLC-89细胞PCNA的表达。结论：大蒜素能抑制SLC-89细胞的增殖,其PCNA的表达比端粒酶活性更容易受到下调。
목적：관찰대산소대인폐선암세포SLC-89증식적영향。방법：불동농도적대산소여SLC-89세포공배양72 h,운용MTT법측정대산소적IC50。장IC50、IC50/2、IC50/4제량적대산소여SLC-89세포공배양72 h후,운용단립중복서렬확증-매련면역흡부법분별측정세포단립매활성,운용류식세포기술측정세포PCNA적표체솔。가입등량생리염수공배양작위대조조。결과：대산소능억제SLC-89세포적증식,기IC50위79.8μg/mL,IC50화IC50/2제량적대산소능현저억제SLC-89세포단립매적활성,IC50、IC50/2、IC50/4제량적대산소도능현저억제SLC-89세포PCNA적표체。결론：대산소능억제SLC-89세포적증식,기PCNA적표체비단립매활성경용역수도하조。
Objective： To investigate the effect of allicin on proliferation of human lung adenocarcinoma cell line （SLC-89）. Methods： SLC-89 cells were cultured under different doses of allicin for 72 h, the 50 % inhibitory concentration （ICs0） was measured by MTY method. SLC-89 cells were then cultured with alfiein at dose IC50, IC50/2, IC2／50/4 for 72 h, respectively, telomerase activities of the treated cells were measured by Telomeric Repeat Amplification Protocol with ELISA （TRAP-ELISA）, and expression of proliferating cell nuclear antigen （PCNA） of the treated cells were tested by flow eytometry. Results： Allicin inhibited proliferation of SLC-89 with IC50 of 79.8 μg/mL. A11icin at dose of IC50 and IC50/2 significantly inhibited telomerase activity of SLC-89 cells, and Alliein at dose of IC50 IC50/2 and IC50/4 significantly inhibited the expression of PCNA of SLC-89 cells. Conclusion： Allicin could inhibit proliferation of SLC-89 cells, compared to the telomerase activity, the expression of PCNA could be down-regulated more easily than by Allicin.