CAJ | 학술논문

研究了茶树油及其组分干预树突状细胞后．采用MTT法检测药物对树突状细胞活力的抑制．并通过倒置显微镜观察细胞形态学变化．以探讨茶树油及其主要组分对小鼠骨髓来源树突状细胞体外生长的影响。结果表明：茶树油、Terpin-4-ol、1．8-Cineole和Terpinolene在实验浓度范围内（0．0021．000μL／mL）对树突状细胞活力均有不同程度的抑制．抑制率与药物浓度呈剂量依赖关系，其中，茶树油对树突状细胞活力抑制率为25．60％（1．000μL／mL）．而同等浓度下的Terpinene-4-ol抑制率为97．20％（P〈0．01），表明Terpinene-4-ol对树突状细胞有较强的细胞毒性：结果还表明，在进行茶树油对树突状细胞活性评价研究时，应控制安全实验浓度在0．310μL／mL以下．
연구료다수유급기조분간예수돌상세포후．채용MTT법검측약물대수돌상세포활력적억제．병통과도치현미경관찰세포형태학변화．이탐토다수유급기주요조분대소서골수래원수돌상세포체외생장적영향。결과표명：다수유、Terpin-4-ol、1．8-Cineole화Terpinolene재실험농도범위내（0．0021．000μL／mL）대수돌상세포활력균유불동정도적억제．억제솔여약물농도정제량의뢰관계，기중，다수유대수돌상세포활력억제솔위25．60％（1．000μL／mL）．이동등농도하적Terpinene-4-ol억제솔위97．20％（P〈0．01），표명Terpinene-4-ol대수돌상세포유교강적세포독성：결과환표명，재진행다수유대수돌상세포활성평개연구시，응공제안전실험농도재0．310μL／mL이하．
The effects of TTO and Terpin-4-ol cytotoxicity towards murine bone marrow-derived dendritic cells were analyzed by the tetrazolium salt reduction （MTT） assay in vitro. The results showed that TTO and Terpin-4-ol exhibited strong cytotoxicity properties towards DC, cell viability decreased in a concentration-dependent manner （0.002-1 μl/ml）. TTO treated DC exhibited significantly decrease at the concentration （0.063-1 μl/ml, P〈0.05）, while Terpin-4-ol at the concentration （0.016-1μl/ml, P〈0.05） much stronger than TTO. The inhibition rate at the concentration of 1μl/ml is 25.60_＋ 5.58% for TTO （P〈0.01）, 97.20± 1.33% for Terpin-4-ol （P〈0.01）, respectively. The safe concentration for the cell test （P〉0.05） is 0.031μl/ml （11.75±7.66%, P〉0.05） for TTO and 0.008μl/ml （4.83±5.28%, P〉 0.05） for Terpin-4-ol, respectively. Terpin-4-ol maybe the most cytotoxicity component of TTO, the results of the present study can be referred to for the appropriate starting dose in cell test and animal toxicity studies.