CAJ | 학술논문

利用6种常用花粉活力测定方法对柱花草花粉活力进行测定．并筛选出各方法的最佳配方。结果表明：以离体萌发法为最佳，最适培养基：10％蔗糖、10％PEG-6000（聚乙二醇）、0．01％心B03、0．03％CaCl2·2H2O、0．01％KNO3，pH6．5，柱花草花粉在该培养基上30℃条件下培养10min，萌发率可达84．03％。0．5％12＋1．5％KI的碘-碘化钾染色效果好，色差明显，较离体萌发法测定值偏高，但差异不显著，可选作简单快速测定法；FDA（荧光素二醋酸脂）染色法、醋酸洋红染色法、无机酸法、TTC（2，3，5-氯代三苯基四氮唑）法均不适合柱花草花粉活力的检测．
이용6충상용화분활력측정방법대주화초화분활력진행측정．병사선출각방법적최가배방。결과표명：이리체맹발법위최가，최괄배양기：10％자당、10％PEG-6000（취을이순）、0．01％심B03、0．03％CaCl2·2H2O、0．01％KNO3，pH6．5，주화초화분재해배양기상30℃조건하배양10min，맹발솔가체84．03％。0．5％12＋1．5％KI적전-전화갑염색효과호，색차명현，교리체맹발법측정치편고，단차이불현저，가선작간단쾌속측정법；FDA（형광소이작산지）염색법、작산양홍염색법、무궤산법、TTC（2，3，5-록대삼분기사담서）법균불괄합주화초화분활력적검측．
Used 6 commonly methods of pollen viability determination on Stylo pollen viability, and screened out their optimal formula. The results showed that pollen germination in vitro method was the best, and the best medium was 10% sucrose, 10% PEG-6000 （polyethylene glycol）, 0.01% HEBO3, 0.03% CaCl2, 2H2O, 0.01% KNO3 and pH 6.5, Stylo pollen germination in this medium at 30℃ for l0 min, and then pollen germination rate were 84.03%. It can be concluded that 0.5% I2 and 1.5% KI components of I-KI staining was the best staining method, because it got no significant difference viability rate to germination in vitro method and can dye fast and clearly. The FDA （fluorescein diacetate） staining, acetocarmine staining, inorganic acid method, TTC （2,3,5-Triphenyltetrazolium chloride ） staining were unsuitable to assay pollen vitality of Stylo.