CAJ | 학술논문

目的：研究黄芪多糖对COLO205人结肠癌细胞株增殖及凋亡的影响。方法：黄芪多糖（25μg/mL、50μg/mL、100μg/mL）与结肠癌细胞共同培养24、48、72h,利用MTT法检测细胞增殖抑制率、免疫组化法检测细胞增殖核抗原（PCNA）表达情况、TUNEL法检测细胞凋亡情况。结果：50μg/mL以上浓度的黄芪多糖对体外培养的COLO205人结肠癌细胞株生长具有抑制作用（抑制率〉30.0%,P〈0.01）,并呈量效和时效关系;与对照组相比,黄芪多糖处理组（50μg/mL、100μg/mL）PCNA表达降低,细胞凋亡增加（凋亡指数〉31.47%,P〈0.01）。结论：黄芪多糖使COLO205人结肠癌细胞增殖减慢,凋亡增加,PCNA表达降低,可能是黄芪多糖抗肿瘤机制之一。
목적：연구황기다당대COLO205인결장암세포주증식급조망적영향。방법：황기다당（25μg/mL、50μg/mL、100μg/mL）여결장암세포공동배양24、48、72h,이용MTT법검측세포증식억제솔、면역조화법검측세포증식핵항원（PCNA）표체정황、TUNEL법검측세포조망정황。결과：50μg/mL이상농도적황기다당대체외배양적COLO205인결장암세포주생장구유억제작용（억제솔〉30.0%,P〈0.01）,병정량효화시효관계;여대조조상비,황기다당처리조（50μg/mL、100μg/mL）PCNA표체강저,세포조망증가（조망지수〉31.47%,P〈0.01）。결론：황기다당사COLO205인결장암세포증식감만,조망증가,PCNA표체강저,가능시황기다당항종류궤제지일。
[Objective] To investigate the effect of astragalus polysaccharides on COLO205 colon cancer cells proliferation and apoptosis. [Method] COLO205 colon cancer cells were co-cultured with various of astragalus polysaccharides for 24h, 48h, 72h respectively, and the rate of cell proliferation inhibition was detected by MTY assay. The levels of PCNA expression were detected by immunohistochemistry method, and the cell apoptosis were detected by TUNEL kit. [Result] Astragalus polysaccharides could effectively inhibit the COLO205 colon cancer ceils proliferation in dose-dependent and time-dependent manner, reduce the level of PCNA expression, and induce cell apoptosis. [Conclusion] Astragalus polysaccharides substantially inhibited COLO205 colon cancer cells proliferation, induced apoptosis and down regulated PCNA expression.