CAJ | 학술논문

采用超声波辅助法提取洋葱皮中黄酮类物质,通过柱层析等方法进行分离纯化,利用高效液相色谱对黄酮类物质进行检测,在超声波辅助萃取洋葱皮中总黄酮的研究中,确定乙醇浓度80%、料液比1∶10、超声时间20min条件下对总黄酮提取率最高。获得总黄酮物质后采用萃取、聚酰胺柱层析进行分离纯化,获得不同浓度乙醇洗脱组分,建立HPLC方法为进样量5μL、检测波长360nm、甲醇∶水（含0.1%冰乙酸）=45∶55、柱温为室温。分离纯化中得到结晶,通过质谱、核磁共振鉴定此晶体物质为槲皮素。
채용초성파보조법제취양총피중황동류물질,통과주층석등방법진행분리순화,이용고효액상색보대황동류물질진행검측,재초성파보조췌취양총피중총황동적연구중,학정을순농도80%、료액비1∶10、초성시간20min조건하대총황동제취솔최고。획득총황동물질후채용췌취、취선알주층석진행분리순화,획득불동농도을순세탈조분,건립HPLC방법위진양량5μL、검측파장360nm、갑순∶수（함0.1%빙을산）=45∶55、주온위실온。분리순화중득도결정,통과질보、핵자공진감정차정체물질위곡피소。
This paper studied the technological process of extracting flavones from onionskin, established a HPLC method for determining the flavones in the extraction of the onionskin. The optimum ultrasonic extraction conditions for flavones were also established which were soak onion for 20min with 80% ethanol concentration and ratio of liquid to solid was l：10. In the study of the HPLC method for determining the flavones, we got the condition of a 5k~L sample injected, detection was carried out at 360 nm, a mixture of methanol and water with 0. 1% glacial acetic acid （45：55） was used as mobile phase. In the course of separation and purification, we got the crystallization with high pure. By the way of MS and NMR, we knew this material was quercetin.