CAJ | 학술논문

目的探讨食管癌患者治疗前后外周血淋巴细胞的变化及其与预后的关系。方法采用流式细胞术（FCM）检测53例食管癌患者治疗前后外周血淋巴细胞水平。结果食管癌患者治疗后与治疗前比较，CD3+细胞水平升高，差异有显著性，其余指标差异无显著性；Ⅲ～Ⅳ期和Ⅰ～Ⅱ期的患者比较，治疗前CD4+和CD3+细胞水平较低，差异有显著性，其余指标及治疗后各项指标差异均无显著性；有淋巴结转移和无淋巴结转移的患者比较，治疗前自然杀伤细胞（NK）水平较高，差异有显著性，其余指标及治疗后各项指标差异均无显著性；有复发或转移与无复发或转移的患者比较，治疗前CD4+细胞水平较低，CD8+、NK、CD19+细胞水平较高，差异有显著性，治疗后CD4+细胞水平较低，CD8+、NK、CD19+细胞水平较高，差异有显著性，CD3+细胞水平差异无显著性。结论CD4+细胞水平减低伴随CD8+、NK和CD19+细胞水平显著升高，可能是评估食管癌治疗后复发或迅速恶化的标志。
목적탐토식관암환자치료전후외주혈림파세포적변화급기여예후적관계。방법채용류식세포술（FCM）검측53례식관암환자치료전후외주혈림파세포수평。결과식관암환자치료후여치료전비교，CD3+세포수평승고，차이유현저성，기여지표차이무현저성；Ⅲ～Ⅳ기화Ⅰ～Ⅱ기적환자비교，치료전CD4+화CD3+세포수평교저，차이유현저성，기여지표급치료후각항지표차이균무현저성；유림파결전이화무림파결전이적환자비교，치료전자연살상세포（NK）수평교고，차이유현저성，기여지표급치료후각항지표차이균무현저성；유복발혹전이여무복발혹전이적환자비교，치료전CD4+세포수평교저，CD8+、NK、CD19+세포수평교고，차이유현저성，치료후CD4+세포수평교저，CD8+、NK、CD19+세포수평교고，차이유현저성，CD3+세포수평차이무현저성。결론CD4+세포수평감저반수CD8+、NK화CD19+세포수평현저승고，가능시평고식관암치료후복발혹신속악화적표지。
Objective To explore the change of peripheral blood lymphocytes in patients with esophageal cancer before and after the treatment, and its relationship with the prognosis. Methods Flow cytometry (FCM) was used to detect the levels of peripheral blood lymphocytes before and after the treatment in patients with esophageal cancer. Results Compared the patients after treatment with those before treatment, the level of CD3+cells was elevated, the difference was statistically signiifcant, there was no statistically signiifcant difference among the rest of the indicators;Compared the patients with stageⅢ~Ⅳ with those with stageⅠ~Ⅱ, the levels of CD4+and CD3+cells were depressed in patients before treatment, the difference was statistically signiifcant, the rest of the indicators and the difference between each index after treatment had no statistical signiifcance;Compared the patients with lymph node metastasis with those without lymph node metastasis, the level of NK was elevated inpatients before treatment, the difference was statistically signiifcant, the rest of the indicators and the difference between each index after treatment had no statistical signiifcance;Compared the patients with recurrence or metastasis with those without recurrence or metastasis, the level of CD4+cells was depressed in patients before and after the treatment ,and the levels of CD8+, NK cell, CD19+cells levels were elevated, the difference was statistically signiifcant, there was no statistically signiifcant difference of CD3+cells. Conclusion The reducing of CD4+ cells along with the increasing of CD8+ , NK and CD19+ cells may be a sign of assessing recurrence and deteriorating rapidly in patients with esophageal cancer after treatment.