玉林师范学院学报
玉林師範學院學報
옥림사범학원학보
Journal of Yulin Teachers College
2013年
5期
73-76
,共4页
毛冬青茎%黄酮%超声波%正交试验
毛鼕青莖%黃酮%超聲波%正交試驗
모동청경%황동%초성파%정교시험
Pubescent Holly stem%flavonoids,ultrasonic wave%orthogonal experiment
目的：筛选乙醇提取毛冬青茎中总黄酮的最佳条件.方法：采用正交试验研究乙醇浓度、料液比、提取时间、提取次数对毛冬青茎中总黄酮提取的影响，用紫外分光光度法测定总黄酮含量.结果：乙醇作为提取溶剂的最佳工艺是乙醇浓度30%，料液比1﹕20，提取3h，提取3次，此条件下吸光值为0.529.结论：乙醇浓度影响最大，其次是提取次数，提取时间、料液比对毛冬青茎中总黄酮提取影响较小。
目的：篩選乙醇提取毛鼕青莖中總黃酮的最佳條件.方法：採用正交試驗研究乙醇濃度、料液比、提取時間、提取次數對毛鼕青莖中總黃酮提取的影響，用紫外分光光度法測定總黃酮含量.結果：乙醇作為提取溶劑的最佳工藝是乙醇濃度30%，料液比1﹕20，提取3h，提取3次，此條件下吸光值為0.529.結論：乙醇濃度影響最大，其次是提取次數，提取時間、料液比對毛鼕青莖中總黃酮提取影響較小。
목적：사선을순제취모동청경중총황동적최가조건.방법：채용정교시험연구을순농도、료액비、제취시간、제취차수대모동청경중총황동제취적영향，용자외분광광도법측정총황동함량.결과：을순작위제취용제적최가공예시을순농도30%，료액비1﹕20，제취3h，제취3차，차조건하흡광치위0.529.결론：을순농도영향최대，기차시제취차수，제취시간、료액비대모동청경중총황동제취영향교소。
Objective:To optimize and establish the extraction of total flavones in Pubescent Holly stem using ethanol as the extraction solvent. Method:Orthogonal experiment was designed with the content of total flavonoids as index, and with ethanol concentration, the ratio of material and solvent, extracting time and frequency as factors. Results:When using ethanol as the extraction solvent, 30%ethanol concentration, solid to liquid ratio of 1:20, extraction time was 3h, extracting 3 times, at this time was the best extraction effect;the OD500 was up to 0.529. Conclusion:The first factor affecting extractor productivity was volume percentage of ethonal. The second and the third ones were extracting frequency and time respectively.
