CAJ | 학술논문

对采于四川省泸县地区的沼水蛙（Hylarana guentheri）线粒体16SrRNA基因进行扩增测序。经3对扩增引物分别扩增及测序获得长度为550bp、300bp、1000bp核苷酸序列，拼接后序列长度1601bp。核苷酸相似性分析表明，序列与Genbank公布的福建的沼水蛙线粒体16SrRNA基因（KF185060）核苷酸序列相似性最高（99.8%）；与越南地区沼水蛙的16S序列相似性也较高（99%~99.7%），无明显种间差异，从遗传上证明沼水蛙为广布物种。同时，与亲源关系较近的水蛙类代表物种构建的系统发育树显示，所有沼水蛙聚为一支系，然后与Rana lateralis互为姊妹群。本文同时也对水蛙类的系统发育关系做了初步讨论。
대채우사천성로현지구적소수와（Hylarana guentheri）선립체16SrRNA기인진행확증측서。경3대확증인물분별확증급측서획득장도위550bp、300bp、1000bp핵감산서렬，병접후서렬장도1601bp。핵감산상사성분석표명，서렬여Genbank공포적복건적소수와선립체16SrRNA기인（KF185060）핵감산서렬상사성최고（99.8%）；여월남지구소수와적16S서렬상사성야교고（99%~99.7%），무명현충간차이，종유전상증명소수와위엄포물충。동시，여친원관계교근적수와류대표물충구건적계통발육수현시，소유소수와취위일지계，연후여Rana lateralis호위자매군。본문동시야대수와류적계통발육관계주료초보토론。
The present paper clones and sequences the mitochondrial 16SrRNA of Hylarana guentheri from Luxian county in Sichuan province. Three nucleotide sequences (550bp, 300bp, 1000bp) have been cloned and sequenced with 3 pairs of primer were assembled into a complete 1601bp contig. The result shows that the sequence was 99.8%identical to that of another H. guentheri sample from Fujian province (KF185060), and 99%-99.7%to the ones from Vietnam. This result proves H. guentheri to be a widespread species geneti-cally. Further, the phylogenetic analysis including available 16S sequences of Hylarana species shows that all H. guentheri sequences clustered together as the sister group to that of Rana lateralis. In addition, the phyloge-netical relationship among Hylarana species has been discussed.