CAJ | 학술논문

目的：观察白细胞介素-10（IL-10）基因修饰对大鼠肝细胞增殖、凋亡的影响，并筛选出表达可能受影响的细胞因子。方法体外培养大鼠肝细胞系BRL ，分为对照组（C组），转染 pcDNA3．0空质粒（P组），转染pcDNA3．0-rIL-10重组质粒（I组）。M T T法检测其增殖，流式细胞法检测其凋亡，抗体芯片法检测其细胞因子的表达。结果转染48 h后，P组较C组增殖减少（ P＝0．000），凋亡增加（ P＝0．000）；I组较P组增殖差别无统计学意义（ P＝0．279），但凋亡明显减少（ P＝0．000）。抗体芯片显示I组较P组除IL-10表达增加之外，血管表皮生长因子（VEGF）的表达下调。结论 IL-10基因治疗肝纤维化中，将肝细胞做为IL-10基因的表达细胞具有可行性。IL-10基因修饰后，肝细胞除了高表达IL-10之外，还通过下调VEGF的表达间接作用。
목적：관찰백세포개소-10（IL-10）기인수식대대서간세포증식、조망적영향，병사선출표체가능수영향적세포인자。방법체외배양대서간세포계BRL ，분위대조조（C조），전염 pcDNA3．0공질립（P조），전염pcDNA3．0-rIL-10중조질립（I조）。M T T법검측기증식，류식세포법검측기조망，항체심편법검측기세포인자적표체。결과전염48 h후，P조교C조증식감소（ P＝0．000），조망증가（ P＝0．000）；I조교P조증식차별무통계학의의（ P＝0．279），단조망명현감소（ P＝0．000）。항체심편현시I조교P조제IL-10표체증가지외，혈관표피생장인자（VEGF）적표체하조。결론 IL-10기인치료간섬유화중，장간세포주위IL-10기인적표체세포구유가행성。IL-10기인수식후，간세포제료고표체IL-10지외，환통과하조VEGF적표체간접작용。
Objective To observe the effect of interleukin-10 gene modification on the prolifera-tion ,apoptosis and cytokine expression of rat hepatocye in vitro . Method BRL cells ,a normal rat hepa-tocyte line cultured in vitro , were divided into three groups ,group Cwas control group ,group Pwere transfected with plasmid pcDNA 3 .0 and group I with plasmid pcDNA 3 .0-rIL-10 .MTT assay ,flow cyto-metric analysis and cytokine antibody arrays were used to detect on the proliferation ,apoptosis and cyto-kine differential expression of BRL cells . Results Transfected with plasmid pcDNA3 .0 ,proliferation of BRL cells was inhibited ,and apoptosis was increased . This increase of apoptosis was attenuated in BRL cells of group I . Compare with group P ,BRL cells of group I showed a lower expression of vascular en-dothelial growth factor ,in addition to higher expression of IL-10 . Conclusion Hepatocytes are possible to be the ideal target cells of IL-10 gene transfer in the IL-10 gene therapy of liver fibrosis . T ransfected with IL-10 gene ,hepatocytes played an inhibitory role on liver fibrogenesis not only by high IL-10 produc-tion ,but also by low regulation on expression of vascular endothelial growth factor probably .