中国血液流变学杂志
中國血液流變學雜誌
중국혈액류변학잡지
CHINESE JOURNAL OF HEMORHEOLOGY
2013年
3期
424-427
,共4页
裴荣全%尹秀华%王全英%赵丽娟%孙茂民
裴榮全%尹秀華%王全英%趙麗娟%孫茂民
배영전%윤수화%왕전영%조려연%손무민
β淀粉样蛋白%D-半乳糖%动物模型%硫氧还蛋白%阿尔茨海默病%细胞凋亡
β澱粉樣蛋白%D-半乳糖%動物模型%硫氧還蛋白%阿爾茨海默病%細胞凋亡
β정분양단백%D-반유당%동물모형%류양환단백%아이자해묵병%세포조망
β-amyloid protein%D-galactose%animal model%thioredoxin%Alzheimer’s disease%cell apoptosis
目的:初步探讨β淀粉样蛋白1~42(Aβ1~42)及D-半乳糖(D-gal)联合构建的复合型阿尔茨海默病(AD)模型,以及模型中硫氧还蛋白与凋亡相关蛋白的研究。方法采用44只2月龄雄性SD大鼠,共分4组,腹腔注射及侧脑室注射生理盐水的假手术组,腹腔注射D-gal及侧脑室注射Aβ1~42分别建立的D-gal组和Aβ1~42组,腹腔注射D-gal联合侧脑室注射Aβ1~42建立的复合AD模型组。免疫组织化学检测脑组织中Trx,细胞色素c(Cyto-c)及Caspase-9表达。结果与对照组相比,Aβ1~42组、D-gal组和复合模型组皮质、海马中Trx的表达下降,Cyto-c和Caspase-9的表达上升。与Aβ1~42组、D-gal组相比,复合模型组皮质、海马中Trx的表达下降;Cyto-c和Caspase-9的表达上升。结论在复合AD模型中神经细胞凋亡,Trx表达下降,而Cyto-c和Caspase-9表达上升。Trx参与神经细胞的凋亡过程。
目的:初步探討β澱粉樣蛋白1~42(Aβ1~42)及D-半乳糖(D-gal)聯閤構建的複閤型阿爾茨海默病(AD)模型,以及模型中硫氧還蛋白與凋亡相關蛋白的研究。方法採用44隻2月齡雄性SD大鼠,共分4組,腹腔註射及側腦室註射生理鹽水的假手術組,腹腔註射D-gal及側腦室註射Aβ1~42分彆建立的D-gal組和Aβ1~42組,腹腔註射D-gal聯閤側腦室註射Aβ1~42建立的複閤AD模型組。免疫組織化學檢測腦組織中Trx,細胞色素c(Cyto-c)及Caspase-9錶達。結果與對照組相比,Aβ1~42組、D-gal組和複閤模型組皮質、海馬中Trx的錶達下降,Cyto-c和Caspase-9的錶達上升。與Aβ1~42組、D-gal組相比,複閤模型組皮質、海馬中Trx的錶達下降;Cyto-c和Caspase-9的錶達上升。結論在複閤AD模型中神經細胞凋亡,Trx錶達下降,而Cyto-c和Caspase-9錶達上升。Trx參與神經細胞的凋亡過程。
목적:초보탐토β정분양단백1~42(Aβ1~42)급D-반유당(D-gal)연합구건적복합형아이자해묵병(AD)모형,이급모형중류양환단백여조망상관단백적연구。방법채용44지2월령웅성SD대서,공분4조,복강주사급측뇌실주사생리염수적가수술조,복강주사D-gal급측뇌실주사Aβ1~42분별건립적D-gal조화Aβ1~42조,복강주사D-gal연합측뇌실주사Aβ1~42건립적복합AD모형조。면역조직화학검측뇌조직중Trx,세포색소c(Cyto-c)급Caspase-9표체。결과여대조조상비,Aβ1~42조、D-gal조화복합모형조피질、해마중Trx적표체하강,Cyto-c화Caspase-9적표체상승。여Aβ1~42조、D-gal조상비,복합모형조피질、해마중Trx적표체하강;Cyto-c화Caspase-9적표체상승。결론재복합AD모형중신경세포조망,Trx표체하강,이Cyto-c화Caspase-9표체상승。Trx삼여신경세포적조망과정。
Objective To investigate the multiple Alzheimer’s disease(AD) animal model established byβAmyloid protein 1~42(Aβ1~42) combined with D-galactose(D-gal) injection.and to investigate thioredoxin and apoptosis-related protein in a multiple AD model.Methods 44 2 months-old SD male rats was divided randomly to 4 group:peritoneal injection and lateral ventricle injection of physiological saline served as sham group,peritoneal injection of D-gal and lateral ventricular injection of Aβ1~42 individually served as a single factor AD model,peritoneal injection of D-gal combined with lateral ventricle injection of Aβ1~42 established a multiple AD animal model.Detect the expression of Trx,cytochrome c(Cyto-c) and Caspase-9 in brain tissue by immunohistochemistry.Results Compared with the sham group,Aβ1~42 group,D-gal group and the multiple model group,the expression of Trx in cortex and hippocampus decreased,the expression of Cyto-c and Caspase-9 increased.And Aβ1~42 group,D-gal group,the multiple model group,the expression of Trx in cortex and hippocampus decreased;the expression of Cyto-c and Caspase-9 increased.Conclusion Trx expression decreased,and Cyto-c and Caspase-9 expression increased in multiple AD model.Trx involved in nerve cell apoptosis.