中国血液流变学杂志
中國血液流變學雜誌
중국혈액류변학잡지
CHINESE JOURNAL OF HEMORHEOLOGY
2013年
3期
413-415
,共3页
姚根宏%张国栋%吴永政
姚根宏%張國棟%吳永政
요근굉%장국동%오영정
脐带造血干细胞%巨核细胞%microRNA
臍帶造血榦細胞%巨覈細胞%microRNA
제대조혈간세포%거핵세포%microRNA
hematopoietic stem cells%megakaryocytes%microRNA
目的:探讨定向诱导造血干细胞分化为巨核细胞和血小板的分子机制。方法将脐带造血干细胞培养后,分离巨核细胞,分别提取造血干细胞和巨核细胞总RNA,进行microRNA芯片分析。结果与造血干细胞相比,巨核细胞中表达上调超过2倍的microRNA有183个,下调超过2倍的microRNA有139个。结论造血干细胞和巨核细胞microRNA表达谱存在差异,尤其是多个microRNA与定向分化巨核细胞相关。
目的:探討定嚮誘導造血榦細胞分化為巨覈細胞和血小闆的分子機製。方法將臍帶造血榦細胞培養後,分離巨覈細胞,分彆提取造血榦細胞和巨覈細胞總RNA,進行microRNA芯片分析。結果與造血榦細胞相比,巨覈細胞中錶達上調超過2倍的microRNA有183箇,下調超過2倍的microRNA有139箇。結論造血榦細胞和巨覈細胞microRNA錶達譜存在差異,尤其是多箇microRNA與定嚮分化巨覈細胞相關。
목적:탐토정향유도조혈간세포분화위거핵세포화혈소판적분자궤제。방법장제대조혈간세포배양후,분리거핵세포,분별제취조혈간세포화거핵세포총RNA,진행microRNA심편분석。결과여조혈간세포상비,거핵세포중표체상조초과2배적microRNA유183개,하조초과2배적microRNA유139개。결론조혈간세포화거핵세포microRNA표체보존재차이,우기시다개microRNA여정향분화거핵세포상관。
Objective To elucidate the mechanism of megakaryocyte development and platelet production from hematopoietic stem cells. Methods CD34+ cells were isolated from umbilical cord blood and cultivated. Megakaryocytes were puriifed from cultivated CD34+cells.Total RNAs from CD34+cells and megakaryocytes were extracted.Microarray analysis was performed to determine differential expressed microRNAs.Results Differential microRNAs were defined as a statistically significant difference with 2 fold or greater change. Compared with hematopoietic stem cells,183 microRNAs showed higher expression and 139 microRNAs were down-regulated in megakaryocytes.Conclusion The differential expressed microRNAs between hematopoietic stem cells and megakaryocytes indicates that some microRNAs play an important role in regulating megakaryocytes differentiation from hematopoietic stem cells.