CAJ | 학술논문

目的：探讨SPARC和Tiam1基因在Burkitt淋巴瘤Raji细胞中的表达及其意义。方法常规细胞培养，取对数生长期的 Raji细胞进行mRNA抽提，采用荧光定量聚合酶链反应（qRQ-PCR）检测SPARC和Tiam1在Raji细胞的mRNA的表达水平。结果Raji细胞中SPARC和Tiam1的mRNA表达水平的CT值均明显高于对照组HUVEC细胞，两两相比，差异均有统计学意义（P＜0.05）；其F值分别为1.473倍和15.434倍。结论SPARC与Tiam1的高表达可能是Burkitt淋巴瘤易出现侵袭转移的重要原因之一。
목적：탐토SPARC화Tiam1기인재Burkitt림파류Raji세포중적표체급기의의。방법상규세포배양，취대수생장기적 Raji세포진행mRNA추제，채용형광정량취합매련반응（qRQ-PCR）검측SPARC화Tiam1재Raji세포적mRNA적표체수평。결과Raji세포중SPARC화Tiam1적mRNA표체수평적CT치균명현고우대조조HUVEC세포，량량상비，차이균유통계학의의（P＜0.05）；기F치분별위1.473배화15.434배。결론SPARC여Tiam1적고표체가능시Burkitt림파류역출현침습전이적중요원인지일。
Objective To investigate the expression and signiifcance of SPARC and Tiam1 genes in Raji cell line of Burkitt’s lymphoma to provide theoretical basis in invasion and metastasis of Burkitt’s lymphoma.Method After cell culture of Raji cell line,and DNA extraction from logarithmic phase cells,the gene expressions of SPARC and Tiam1 were detected by real-time fluorescent quantitative PCR.Results The gene expressions of SPARC and Tiam1 were up-regulated in Raji cell line,the differences were statistically significant(P<0.05),F=1.473 and 15.434.Conclusion The gene expressions of SPARC and Tiam1 may play an important role in invasion and metastasis of Burkitt’s lymphoma.