CAJ | 학술논문

分析简单节杆菌乙醇耐性差异菌株的生长特性，以静息细胞为对象，考察乙醇处理对菌体细胞存活率的影响，进一步分析细胞糖代谢能力、细胞膜电位、细胞超显微结构和通透性的变化．结果发现：乙醇体积分数在0%～10%的范围内，突变菌株UV15X1-2比出发菌株TCCC 11037表现出更好的生长特性；6%和10%乙醇处理后，突变菌株UV15X1-2的细胞存活率比出发菌株TCCC 11037分别高出19.2%和18.3%，表现出更好的乙醇耐受性；进一步研究发现，6%乙醇引起出发菌株TCCC 11037糖代谢能力和膜电位的明显下降，细胞完整性遭到明显破坏，而对于突变菌株UV15X1-2，这些变化在乙醇体积分数为10%的条件下出现．此外，通过测定FDA的相对荧光强度和细胞蛋白质及核酸的泄漏量，表明一定体积分数的乙醇处理能显著提高简单节杆菌的细胞通透性．
분석간단절간균을순내성차이균주적생장특성，이정식세포위대상，고찰을순처리대균체세포존활솔적영향，진일보분석세포당대사능력、세포막전위、세포초현미결구화통투성적변화．결과발현：을순체적분수재0%～10%적범위내，돌변균주UV15X1-2비출발균주TCCC 11037표현출경호적생장특성；6%화10%을순처리후，돌변균주UV15X1-2적세포존활솔비출발균주TCCC 11037분별고출19.2%화18.3%，표현출경호적을순내수성；진일보연구발현，6%을순인기출발균주TCCC 11037당대사능력화막전위적명현하강，세포완정성조도명현파배，이대우돌변균주UV15X1-2，저사변화재을순체적분수위10%적조건하출현．차외，통과측정FDA적상대형광강도화세포단백질급핵산적설루량，표명일정체적분수적을순처리능현저제고간단절간균적세포통투성．
The growing properties of Arthrobacter Simplex strains with different organic solvent tolerance were studied. Using resting cells as materials,the effects of ethanol on the cell survival,sugar metabolic activity,membrane potential,cell ultrastructure and permeability were investigated. The results showed that the growing properties of UV15X1-2 were better than those of TCCC 11037 within the range of ethanol concentration(0%-10%). After the treatment with 6% and 10% of ethanol,the cell survival rate of UV15X1-2 was 19.2%and 18.3%higher compared with TCCC 11037,which suggested that UV15X1-2 presented higher tolerance toward ethanol. As for TCCC 11037,sugar metabolic activities and membrane potential decreased rapidly,and the cell integrity was significantly damaged with 6%of ethanol,but similar effects on cell properties and structure were not observed until UV15X1-2 was treated with 10%of ethanol. On the other hand,the relative fluorescence intensity of FDA and the protein/nucleic acid leakage revealed that ethanol could enhance the permeability of the Arthrobacter simplex cell significantly.