吉林农业大学学报
吉林農業大學學報
길임농업대학학보
JOURNAL OF JILIN AGRICUL TURAL UNIVERSITY
2013年
6期
708-715
,共8页
杨扬%綦天华%胡静%杜金%吴宗翰%宋慧
楊颺%綦天華%鬍靜%杜金%吳宗翰%宋慧
양양%기천화%호정%두금%오종한%송혜
小刺猴头菌丝体多糖%分离纯化%抗氧化%抗肿瘤
小刺猴頭菌絲體多糖%分離純化%抗氧化%抗腫瘤
소자후두균사체다당%분리순화%항양화%항종류
polysaccharides for the mycelium of Hericium caput-medusae%isolation and purification%an-tioxidant activity%anti-tumor activity
试验研究了小刺猴头菌丝体多糖的结构性质、抗氧化活性和抗肿瘤活性。对小刺猴头菌丝体采用水提和碱提2种方法获得粗多糖,进行 DEAE-cellulose,Sephadex G-75和 Sepharose CL-6B 柱层析进一步纯化,获得的多糖组分经纯度检验之后进行多糖性质的研究。结果表明:小刺猴头菌丝体水提多糖主要含有葡萄糖和甘露糖;碱提多糖主要含有葡萄糖和半乳糖;小刺猴头菌丝体多糖具有一定的抗氧化活性和抗肿瘤活性。
試驗研究瞭小刺猴頭菌絲體多糖的結構性質、抗氧化活性和抗腫瘤活性。對小刺猴頭菌絲體採用水提和堿提2種方法穫得粗多糖,進行 DEAE-cellulose,Sephadex G-75和 Sepharose CL-6B 柱層析進一步純化,穫得的多糖組分經純度檢驗之後進行多糖性質的研究。結果錶明:小刺猴頭菌絲體水提多糖主要含有葡萄糖和甘露糖;堿提多糖主要含有葡萄糖和半乳糖;小刺猴頭菌絲體多糖具有一定的抗氧化活性和抗腫瘤活性。
시험연구료소자후두균사체다당적결구성질、항양화활성화항종류활성。대소자후두균사체채용수제화감제2충방법획득조다당,진행 DEAE-cellulose,Sephadex G-75화 Sepharose CL-6B 주층석진일보순화,획득적다당조분경순도검험지후진행다당성질적연구。결과표명:소자후두균사체수제다당주요함유포도당화감로당;감제다당주요함유포도당화반유당;소자후두균사체다당구유일정적항양화활성화항종류활성。
Polysaccharides for the mycelium of Hericium caput-medusae were studied on the structural properties,antioxidant activity and anti-tumor activity.Polysaccharides for the mycelium of Hericium ca-put-medusae were extracted by water extraction and alkali extraction.The coarse polysaccharides were pu-rified by DEAE-cellulose,Sephadex G75 and Sepharose CL-6B gel column chromatography methods.The pure polysaccharide was studied on properties after the purity test.The results showed that water extraction polysaccharides for the mycelium of Hericium caput-medusae mainly contained glucose and mannose;alka-li extraction polysaccharides mainly contained glucose and galactose.Polysaccharides for the mycelium of Hericium caput-medusae have certain antioxidant activity and antitumor activity.