激光杂志
激光雜誌
격광잡지
LASER JOURNAL
2013年
6期
113-114
,共2页
鼻咽癌%CCL18%迁移
鼻嚥癌%CCL18%遷移
비인암%CCL18%천이
nasopharyngeal carcinoma%CCL18%metastasis
提目的：探讨趋化因子配体18（CCL18）在鼻咽癌患者血浆中的表达水平及检测在体外对人鼻咽癌细胞株增殖和迁移能力的影响。方法：收集到重庆医科大学附属第二医院就诊并病理诊断为鼻咽癌患者37例，在未接受任何治疗前留取血液标本，另取20例健康体检者外周血液标本作为正常人群对照。以酶联免疫吸附测定（ELIAS ）方法检测血浆中CCL18的表达水平。体外实验以人鼻咽癌细胞株HNE-1为研究对象，用噻唑蓝（MTT）比色法检测重组CCL18对细胞增殖能力的影响，用Transwell体外迁移系统检测CCL18对鼻咽癌细胞的趋化作用。结果：鼻咽癌患者血浆中CCL18的表达水平明显高于健康体检者（P＜0．0001），伴有颈部淋巴结转移的鼻咽癌患者较未发生颈部淋巴结转移者血浆中CCL18的表达水平显著升高（P＜0．05）。CCL18因子在体外对鼻咽癌细胞无明显促进增殖作用（P＞0．05）。 CCL18对鼻咽癌细胞具有明显促进迁移趋化作用，并呈现浓度依赖性。结论：CCL18是鼻咽癌的生物学标志物，其表达与鼻咽癌的侵袭转移相关，对鼻咽癌的诊断和预后判断有一定临床意义。
提目的：探討趨化因子配體18（CCL18）在鼻嚥癌患者血漿中的錶達水平及檢測在體外對人鼻嚥癌細胞株增殖和遷移能力的影響。方法：收集到重慶醫科大學附屬第二醫院就診併病理診斷為鼻嚥癌患者37例，在未接受任何治療前留取血液標本，另取20例健康體檢者外週血液標本作為正常人群對照。以酶聯免疫吸附測定（ELIAS ）方法檢測血漿中CCL18的錶達水平。體外實驗以人鼻嚥癌細胞株HNE-1為研究對象，用噻唑藍（MTT）比色法檢測重組CCL18對細胞增殖能力的影響，用Transwell體外遷移繫統檢測CCL18對鼻嚥癌細胞的趨化作用。結果：鼻嚥癌患者血漿中CCL18的錶達水平明顯高于健康體檢者（P＜0．0001），伴有頸部淋巴結轉移的鼻嚥癌患者較未髮生頸部淋巴結轉移者血漿中CCL18的錶達水平顯著升高（P＜0．05）。CCL18因子在體外對鼻嚥癌細胞無明顯促進增殖作用（P＞0．05）。 CCL18對鼻嚥癌細胞具有明顯促進遷移趨化作用，併呈現濃度依賴性。結論：CCL18是鼻嚥癌的生物學標誌物，其錶達與鼻嚥癌的侵襲轉移相關，對鼻嚥癌的診斷和預後判斷有一定臨床意義。
제목적：탐토추화인자배체18（CCL18）재비인암환자혈장중적표체수평급검측재체외대인비인암세포주증식화천이능력적영향。방법：수집도중경의과대학부속제이의원취진병병리진단위비인암환자37례，재미접수임하치료전류취혈액표본，령취20례건강체검자외주혈액표본작위정상인군대조。이매련면역흡부측정（ELIAS ）방법검측혈장중CCL18적표체수평。체외실험이인비인암세포주HNE-1위연구대상，용새서람（MTT）비색법검측중조CCL18대세포증식능력적영향，용Transwell체외천이계통검측CCL18대비인암세포적추화작용。결과：비인암환자혈장중CCL18적표체수평명현고우건강체검자（P＜0．0001），반유경부림파결전이적비인암환자교미발생경부림파결전이자혈장중CCL18적표체수평현저승고（P＜0．05）。CCL18인자재체외대비인암세포무명현촉진증식작용（P＞0．05）。 CCL18대비인암세포구유명현촉진천이추화작용，병정현농도의뢰성。결론：CCL18시비인암적생물학표지물，기표체여비인암적침습전이상관，대비인암적진단화예후판단유일정림상의의。
Objective :To investigate the express level of CC -chemokine Ligand 18(CCL18)in patients with nasopharyngeal carcinoma (NPC)and explore the role of CCL18 in the proliferation and migration of tumor cell in vitro .Method 37 patients with pathologic al proved NPC were enrolled before any treatments .20 healthy people were enrolled as normal controls .The CCL18 in plasms was detected by enzyme -linked immune sorbent assay (ELISA ) .In vivo experiment ,after addition of CCL18 ,the changes of NPC cell (HNE-1) growth were observed by MTT .The changes of cell migration were measured by Transwell .Result The level of CCL18 in the NPC patients’ plasms was significantly higher than that in healthy people (p<0 .0001) .The number of migration cells of NPC cell was increased higher after CCL18 treatment (higher concentration of 40ng/ml) .The proliferation of cell had no effect as well as migration .Conclusion CCL18 is a biomarker of NPC ,and has correlation to the metastasis of NPC .CCL18 play a clinical significance in diagnosis and prognosis of NPC .
