CAJ | 학술논문

目的：筛选胎儿宫内发育迟缓大鼠血清中差异表达的蛋白质，寻找与胎儿宫内发育迟缓相关的疾病标志物。方法收集大鼠疾病正常组、IUGR组血浆各3例；TCA法沉淀血清蛋白，建立疾病组和正常组蛋白质的双向凝胶电泳图谱；Image Master分析软件分析差异蛋白质；飞行时间质谱（MALDI-TOF-MS）获得差异蛋白肽指纹图谱，MASCOT引擎搜索蛋白质种类。结果鉴定出个差异蛋白点3个，分别为：IGF-Ⅰ、IGF-Ⅱ、IGFBP-3。结论 IGF-Ⅰ、IGF-Ⅱ、IGFBP-3，可能是胎儿宫内发育迟缓疾病诊断的生物标志物。
목적：사선태인궁내발육지완대서혈청중차이표체적단백질，심조여태인궁내발육지완상관적질병표지물。방법수집대서질병정상조、IUGR조혈장각3례；TCA법침정혈청단백，건립질병조화정상조단백질적쌍향응효전영도보；Image Master분석연건분석차이단백질；비행시간질보（MALDI-TOF-MS）획득차이단백태지문도보，MASCOT인경수색단백질충류。결과감정출개차이단백점3개，분별위：IGF-Ⅰ、IGF-Ⅱ、IGFBP-3。결론 IGF-Ⅰ、IGF-Ⅱ、IGFBP-3，가능시태인궁내발육지완질병진단적생물표지물。
Objective Differential serum proteins of rat with Intrauterine Growth Retardation were identificated with proteomics technology,and associated disease markers of intrauterine growth retardation were found.Method 3 cases rats plasma of normal group and IUGR group were collected,respectively.After serum proteins were extracted with TCA precipitation method,We established proteins Image of two-dimensional gel electrophoresis of IUGR group and normal group.Differential proteins were screened with Image Master software,and peptide mass finger-printing were obtained by MALDI-TOF-MS,then database were searched through the Mascot data engine,and protein identification results were obtained.Results 3 differential protein spots were identificatied.Conclusion IGF-Ⅰ,IGF-Ⅱ,IGFBP-3 are possible bio-markers of In-trauterine Growth Retardation diagnosis.