CAJ | 학술논문

目的：通过观察肿瘤坏死因子-α（tumor necrosis factor-α，TNF-α）变化情况，评价犬高危穿透性角膜移植后RAPA滴眼液抗免疫排斥反应效果。方法选择36只健康犬建立角膜高危病理模型后进行高危穿透性角膜移植手术，并分组用复方氯化钠或1％RAPA点眼，4次/d，18只未手术犬为空白对照组。同时进行外周血和角膜组织中TNF-α含量检测。结果术后7～42 d复方氯化钠组和治疗组TNF-α浓度明显比对照组高（P＜0.01），在术后28 d治疗组TNF-α浓度逐渐降低，复方氯化钠组仍维持较高含量。结论 RAPA滴眼液能降低角膜移植手术后TNF-α含量，有效抗免疫排斥反应。
목적：통과관찰종류배사인자-α（tumor necrosis factor-α，TNF-α）변화정황，평개견고위천투성각막이식후RAPA적안액항면역배척반응효과。방법선택36지건강견건립각막고위병리모형후진행고위천투성각막이식수술，병분조용복방록화납혹1％RAPA점안，4차/d，18지미수술견위공백대조조。동시진행외주혈화각막조직중TNF-α함량검측。결과술후7～42 d복방록화납조화치료조TNF-α농도명현비대조조고（P＜0.01），재술후28 d치료조TNF-α농도축점강저，복방록화납조잉유지교고함량。결론 RAPA적안액능강저각막이식수술후TNF-α함량，유효항면역배척반응。
Objective Observe the Changes of TNF-αby 1%RAPA eye drops after high-risk penetrativity penetrating keratoplasty in canine, evaluate the effect of coping immune rejection of RAPA eye drops.Methods Thirty-six healthy local dogs were chosen as participants to estab-lish high-risk pathologic model,and then dealed with high-risk penetrativity penetrating keratoplasty,after grouping,CoNacl or 1%RAPA eye drops were used 4 times in each day.Meanwhile,the content of TNF-αin Peripheral blood and corneal tissue were measured.Results the concentration of TNF-αin CoNacl group and treatment group were obviously higher than control group(P<0.01)on 7th ~42th day after PKP,and on 28th day,the concentration of TNF-αin treatment group was gradually reduced,but in CoNacl group,it was still high.Conclu-sion RAPA could reduce the content of TNF-αafter high-risk penetrativity penetrating keratoplasty and played an effective role of coping im-mune rejection.