CAJ | 학술논문

目的：观察肾康丸治疗对2型糖尿病（T2DM）大鼠肾组织TRAIL系统表达的影响，探讨其肾脏保护作用机制。方法：建立T2DM大鼠模型。成模后将大鼠随机分为2组：模型对照组（DM）、肾康丸组（DS）；另设正常对照组（C）。分组干预，全自动生化仪检测高密度脂蛋白胆固醇（FBG）、总胆固醇（TC）及空腹血糖（FBG）（HDL-C）；ELISA法检测尿微量白蛋白α1-MG（Uα1-MG）、24 h尿微量白蛋白（24h UmAlb），Q-RT-PCR法检测TRAIL、DR4、DcR2mRNA在肾组织表达，HE染色法观察大鼠肾组织病理变化。结果：随着实验时间的延长，DM组FBG及TC升高，HDL-C降低，而DS组FBG及TC降低，HDL-C升高；与C组相比，第4周、第8周DM组大鼠Uα1-MG、24h Um-Alb均显著升高（P＜0.01，P＜0.01），肾组织DcR2 mRNA表达显著增强（P＜0.01）；而TRAIL、DR4 mRNA表达显著减弱（P＜0.01），并且DM组大鼠出现了肾脏组织病理损害；DS 组各指标均呈相反改变，肾脏病理损害减轻。结论：肾康丸通过影响TRAIL系统的表达，对T2DM大鼠具有显著的肾保护作用。
목적：관찰신강환치료대2형당뇨병（T2DM）대서신조직TRAIL계통표체적영향，탐토기신장보호작용궤제。방법：건립T2DM대서모형。성모후장대서수궤분위2조：모형대조조（DM）、신강환조（DS）；령설정상대조조（C）。분조간예，전자동생화의검측고밀도지단백담고순（FBG）、총담고순（TC）급공복혈당（FBG）（HDL-C）；ELISA법검측뇨미량백단백α1-MG（Uα1-MG）、24 h뇨미량백단백（24h UmAlb），Q-RT-PCR법검측TRAIL、DR4、DcR2mRNA재신조직표체，HE염색법관찰대서신조직병리변화。결과：수착실험시간적연장，DM조FBG급TC승고，HDL-C강저，이DS조FBG급TC강저，HDL-C승고；여C조상비，제4주、제8주DM조대서Uα1-MG、24h Um-Alb균현저승고（P＜0.01，P＜0.01），신조직DcR2 mRNA표체현저증강（P＜0.01）；이TRAIL、DR4 mRNA표체현저감약（P＜0.01），병차DM조대서출현료신장조직병리손해；DS 조각지표균정상반개변，신장병리손해감경。결론：신강환통과영향TRAIL계통적표체，대T2DM대서구유현저적신보호작용。
Aim:To explore the effect of Shenkangwan on the expression of tumor necrosis factor-relat-ed apoptosis-inducing ligand system(TRAIL)in the kidneys of type 2 diabetes rats.Methods:The rat models of type 2 diabetes were randomly divided into diabetic model group (DM)and Shenkangwan treat-ment group (DS),with the rats fed with normal chow as the control group (C).After treatment,The FBG,TC and HDL-C were detected by automatic biochemical analyzer.The levels of urine α1-MG (Uα1-MG)and 24 hour urine mAlb (24h UmAlb)were detected by ELISA,and the expressions of TRAIL,death receptor 4 (DR4 )and decoy receptor 2 (DcR2 )protein and mRNA in the kidney were measured by immune histochemistry and quantitative real-time PCR(Q-RT-PCR),Then we investigated their renal pathological change under optical microscope with the coloration method of Hematoxylin and E-osin(HE).Results:The levels of FBG and TC increased while HDL-C reduced in the DMgroup,these changes were all reversed after treatment with Shenkangwan.Compared with C group,the levels of Uα1-MG,24h UmAlb,and the renal expressions of DcR2 protein and mRNA increased significantly (P <0.01 );however,the expressions of TRAIL and DR4 protein and mRNA(P<0.01 )reduced significantly and came forth about the renal pathological lesion in the DM group.These changes were all reversed and the renal pathological lesion was mitigated after treatment with Shenkangwan.Conclusion:Shenkangwan have significant renal protection on T2DMrats,its mechanisms may be the effects on expression of TRAIL system.