CAJ | 학술논문

为纯化紫苏籽油中的α-亚麻酸,将传统的低温冷冻结晶法和尿素包合法有机结合,对纯化工艺进行优化.结果表明：紫苏籽油在V（无水乙醇）∶V（丙酮）=1∶1混合溶剂,粗品∶尿素∶混合溶剂=2∶1∶10,冷藏时间为36 h时,析出的晶体最长、用时最短.采用筛选的溶剂和优化的工艺条件可大大提高晶体析出量、缩短晶体的析出时间,使纯化后紫苏籽油中的α-亚麻酸从原来的304 mg/mL提高到644 mg/mL.
위순화자소자유중적α-아마산,장전통적저온냉동결정법화뇨소포합법유궤결합,대순화공예진행우화.결과표명：자소자유재V（무수을순）∶V（병동）=1∶1혼합용제,조품∶뇨소∶혼합용제=2∶1∶10,랭장시간위36 h시,석출적정체최장、용시최단.채용사선적용제화우화적공예조건가대대제고정체석출량、축단정체적석출시간,사순화후자소자유중적α-아마산종원래적304 mg/mL제고도644 mg/mL.
In order to purify the α-linolenic acid in the Perilla frutescens seed oil,this studying combines traditional hypothermia frozen crystallization and urea adduction.Through purification process,it is concluded that the longest crystal can be separated out from P. frutescens seed oil in the shortest time under the ratio of 1 to 1 for ethanol ∶ acetone and ration of 2 to 1 to 10 for crude∶urea∶ethanol and acetone mixed solvent.The results showed that crystal deposition amount can be enhanced and precipitation period of crystal can be shortened tremendously by applying filtered solvent and optimized processing conditions.And the α-linolenic acid in the purified P. frutescens seed oil is increased from 304 mg/mL to 644 mg/mL.