国际沙棘研究与开发
國際沙棘研究與開髮
국제사극연구여개발
THE GLOBAL SEABUCKTHORN RESEARCH AND DEVELOPMENT
2014年
1期
1-5
,共5页
张吉科%林美珍%张小民%王云丽
張吉科%林美珍%張小民%王雲麗
장길과%림미진%장소민%왕운려
沙棘叶%提取物%提取方法%提取率%茶多酚%儿茶素%芦丁%槲皮素%维生素C
沙棘葉%提取物%提取方法%提取率%茶多酚%兒茶素%蘆丁%槲皮素%維生素C
사극협%제취물%제취방법%제취솔%다다분%인다소%호정%곡피소%유생소C
seabuckthorn leaves%extrative%extraction method%extraction rate%tea polyphenols%catechin%tutin%quercetin%Vitamin C
将沙棘叶粉碎,在料液比为1:20,提取温度60℃的相同条件下,分别用水和60%乙醇连续3次提取沙棘叶,每次提取2h。研究了沙棘叶中茶多酚、儿茶素、芦丁、槲皮素和维生素 C的提取量和提取率。结果表明,除茶多酚外,醇提优于水提。连续3次提取优于一次提取。沙棘叶细胞中这5种成分,按极性大小,溶出量和溶出速率是:儿茶素>茶多酚>芦丁≈维生素c>槲皮素。它们在沙棘叶中的含量,茶多酚(包括儿茶素)含量最多,其次是黄酮类(芦丁和槲皮素)和维生素C。茶多酚的含量高于黄酮和维生素C10倍以上。而芦丁的含量又高于槲皮素约4.3~4.5倍。
將沙棘葉粉碎,在料液比為1:20,提取溫度60℃的相同條件下,分彆用水和60%乙醇連續3次提取沙棘葉,每次提取2h。研究瞭沙棘葉中茶多酚、兒茶素、蘆丁、槲皮素和維生素 C的提取量和提取率。結果錶明,除茶多酚外,醇提優于水提。連續3次提取優于一次提取。沙棘葉細胞中這5種成分,按極性大小,溶齣量和溶齣速率是:兒茶素>茶多酚>蘆丁≈維生素c>槲皮素。它們在沙棘葉中的含量,茶多酚(包括兒茶素)含量最多,其次是黃酮類(蘆丁和槲皮素)和維生素C。茶多酚的含量高于黃酮和維生素C10倍以上。而蘆丁的含量又高于槲皮素約4.3~4.5倍。
장사극협분쇄,재료액비위1:20,제취온도60℃적상동조건하,분별용수화60%을순련속3차제취사극협,매차제취2h。연구료사극협중다다분、인다소、호정、곡피소화유생소 C적제취량화제취솔。결과표명,제다다분외,순제우우수제。련속3차제취우우일차제취。사극협세포중저5충성분,안겁성대소,용출량화용출속솔시:인다소>다다분>호정≈유생소c>곡피소。타문재사극협중적함량,다다분(포괄인다소)함량최다,기차시황동류(호정화곡피소)화유생소C。다다분적함량고우황동화유생소C10배이상。이호정적함량우고우곡피소약4.3~4.5배。
The crushed seabuckthorn leaves extracted 3 times and each 2 hr with water and 60% etha-nol under the same conditions of solid-liquid ratio of 1:20 and extraction temperature of 60 ℃,the ex-traction amount and extraction rate of tea polyphenols, catechin, rutin, quercetin and vitamin c have been extracted and studied. The result shows that except the tea polyphenols, other components extrac-tion with alcohol extraction is better thanwith water. Extraction by 3 times is better than only one time. According to dissolubility of polarity, amount of dissolution and the dissolution rate, the ranking of these five components is: Catechin > tea polyphenols > rutin > quercetin > vitamin c. Their content in the seabuckthorn leaf, the content of tea polyphenols, including catechins is the most, followed by flavonoids, including rutin and quercetin and vitamin C. Tea polyphenols content is higher than the fla-vonoids and vitamin C about 10 times. Rutin content is higher than that of quercetin is about 4.3-4.5 times.