中南民族大学学报：自然科学版
中南民族大學學報：自然科學版
중남민족대학학보：자연과학판
Journal of South-Central University for Nationalities
2012年
2期
42-44,70
,共4页
余光辉%梅刘娟%余文卉%游文
餘光輝%梅劉娟%餘文卉%遊文
여광휘%매류연%여문훼%유문
愈伤诱导%组织培养%光兼养型%烟草
愈傷誘導%組織培養%光兼養型%煙草
유상유도%조직배양%광겸양형%연초
callus induction%tissue culture%photoautotrophic facultativity%Nicotiana tabacum
以烟草SR1品种的4周龄叶片为外植体,使用MS＋2 mg/L 2,4 D（2,4-二氯苯氧乙酸）＋0.25mg/L KT（6-糖基氨基嘌呤）＋3%蔗糖＋0.8%琼脂的固体培养基成功诱导了叶片的愈伤组织.通过改变蔗糖浓度和激素配比,对兼性愈伤组织的培养基进行了筛选.结果表明：在500 lxs光照下,蔗糖浓度由3%降至1.5%时,愈伤组织有变绿趋势;蔗糖浓度〈1.5%时,愈伤组织容易出现褐化.2 mg/L NAA（α-萘乙酸）＋0.25 mg/L KT的激素组合可有效诱导愈伤组织的光合兼养,而单一植物激素如NAA和6-BA（6-苄氨基腺嘌呤）,不是理想的诱导配方.
以煙草SR1品種的4週齡葉片為外植體,使用MS＋2 mg/L 2,4 D（2,4-二氯苯氧乙痠）＋0.25mg/L KT（6-糖基氨基嘌呤）＋3%蔗糖＋0.8%瓊脂的固體培養基成功誘導瞭葉片的愈傷組織.通過改變蔗糖濃度和激素配比,對兼性愈傷組織的培養基進行瞭篩選.結果錶明：在500 lxs光照下,蔗糖濃度由3%降至1.5%時,愈傷組織有變綠趨勢;蔗糖濃度〈1.5%時,愈傷組織容易齣現褐化.2 mg/L NAA（α-萘乙痠）＋0.25 mg/L KT的激素組閤可有效誘導愈傷組織的光閤兼養,而單一植物激素如NAA和6-BA（6-芐氨基腺嘌呤）,不是理想的誘導配方.
이연초SR1품충적4주령협편위외식체,사용MS＋2 mg/L 2,4 D（2,4-이록분양을산）＋0.25mg/L KT（6-당기안기표령）＋3%자당＋0.8%경지적고체배양기성공유도료협편적유상조직.통과개변자당농도화격소배비,대겸성유상조직적배양기진행료사선.결과표명：재500 lxs광조하,자당농도유3%강지1.5%시,유상조직유변록추세;자당농도〈1.5%시,유상조직용역출현갈화.2 mg/L NAA（α-내을산）＋0.25 mg/L KT적격소조합가유효유도유상조직적광합겸양,이단일식물격소여NAA화6-BA（6-변안기선표령）,불시이상적유도배방.
In this study, 4 weeks of tobacco cultuivar-SR1 leaves were used as the explants and solid culuter medium containing MS ＋ 2 mg/L 2,4-D （ 2,4-dichlorophenoxy ） ＋ 0.25 mg/L KT （kinetin） ＋ 3 % sucrose ＋ 0.8 % agar was employed to successfully induce the callus formation. Then culture mediums aiming for photoautotrophic facultative callus culture were screened by altering the sucrose concentrations and hormone combinations. Under the irradiation of 500 lxs light intensity, when the concentration of sucrose was reduced from 3% to 1. 5%, the callus tissue was found to increasingly turn green. However, when the concentration of sucrose was reduced to below 1.5% , it easily turned brown. The combination of 2 mg/L NAA （1-naphthlcetic） with 0. 25 mg/L KT can effectively induce the production of photoautotrophic facultative callus, whereas the unique plant hormone, such as NAA or 6-BA （6-benzylaminopurine） can not be used as the ideal medium for such induction.
