CAJ | 학술논문

为研究玉柏石松提取物26-失碳-8-氧代-α-芒柄花萜醇（26-NO-Ono）对成骨细胞活性的影响,采用MTT检测不同浓度26-NO-Ono（3.33,6.66,13.32,26.64μmol/L）对成骨细胞增殖率,碱性磷酸酶（ALP）试剂盒检测成骨细胞内ALP活性,荧光定量PCR检测成骨细胞骨相关基因表达.结果表明：26-NO-Ono给药1d可促进成骨细胞增殖,给药3d可促进成骨细胞ALP活性.26-NO-Ono处理3d和9d会抑制骨涎蛋白（BSP）、I型胶原蛋白（Col-I）以及骨钙素蛋白（OCN）的基因表达;处理6d会促进上述基因的表达.26-NO-Ono长期处理（6d和9d）可以抑制骨桥蛋白（OPN）的基因表达,说明26-NO-Ono对成骨细胞的成骨活性的影响呈时间依赖性,剂量依赖性和细胞分化状态依赖性.
위연구옥백석송제취물26-실탄-8-양대-α-망병화첩순（26-NO-Ono）대성골세포활성적영향,채용MTT검측불동농도26-NO-Ono（3.33,6.66,13.32,26.64μmol/L）대성골세포증식솔,감성린산매（ALP）시제합검측성골세포내ALP활성,형광정량PCR검측성골세포골상관기인표체.결과표명：26-NO-Ono급약1d가촉진성골세포증식,급약3d가촉진성골세포ALP활성.26-NO-Ono처리3d화9d회억제골연단백（BSP）、I형효원단백（Col-I）이급골개소단백（OCN）적기인표체;처리6d회촉진상술기인적표체.26-NO-Ono장기처리（6d화9d）가이억제골교단백（OPN）적기인표체,설명26-NO-Ono대성골세포적성골활성적영향정시간의뢰성,제량의뢰성화세포분화상태의뢰성.
To study the effects of 26-nor-8-oxo-α-onocerin （26-NO-Ono）, an extract from Lycopodium obscurum L. , on the activity of cultured osteoblasts in vitro, different concentrations （3.33, 6.66, 13.32, 26. 641μmol/L） of 26-NO-Ono were given to cultured osteblasts. MTF assay, alkaline phosphate （ALP） kit, real-time PCR were applied to measure the proliferation rate, activity of ALP and expression of bone-related genes in treated osteoblasts. Results showed that treatment with 26-NO-Ono for 1 d can promote the osteoblast proliferation rate and 3 d can enhance the ALP activity. Treatment with 26-NO-Ono for 3 d and 9 d can inhibit the expression of bone-related genes such as BSP, Col- I and OCN in osteoblasts, while treatment for 6 d can stimulate the above 3 genes. Meanwhile, treatment with 26-NO-Ono for 6 d and 9 d could inhibit the expression of OPN gene. Our results indicated that the effect of 26-NO-Ono on osteogenic activity of osteoblast is time-dependent, concentration-dependent and differentiation state-dependent.