CAJ | 학술논문

以双-[-6-氧-（-2-间羧基苯磺酰基-丁二酸-1,4-单酯-4-）-]-β-环糊精（β-CD-B2）为手性添加剂在高效毛细管电泳（HPCE）中分离布洛芬,考察了缓冲溶液硼砂的浓度、pH值,手性添加剂β-CD-B2的浓度,分离电压对布洛芬手性异构体分离的影响.结果8min内实现了对映体的基线分离,最佳分离条件下分离度Rs为4.26.布洛芬对映体分离的前峰线性范围为0.0538～0.942g/L,后峰线性范围为0.178～0.942g/L.在分离布洛芬的最佳条件下,可同时分离布比卡因和布洛芬2种手性药物的混合溶液.
이쌍-[-6-양-（-2-간최기분광선기-정이산-1,4-단지-4-）-]-β-배호정（β-CD-B2）위수성첨가제재고효모세관전영（HPCE）중분리포락분,고찰료완충용액붕사적농도、pH치,수성첨가제β-CD-B2적농도,분리전압대포락분수성이구체분리적영향.결과8min내실현료대영체적기선분리,최가분리조건하분리도Rs위4.26.포락분대영체분리적전봉선성범위위0.0538～0.942g/L,후봉선성범위위0.178～0.942g/L.재분리포락분적최가조건하,가동시분리포비잡인화포락분2충수성약물적혼합용액.
Chiral separation of ibuprofen was investigated by bis- L -6-oxygen - （ -2-m-carboxyl phenylsulfonyl - succinic acid 1,4 - ester -4 -）-]-β-cyclodextrin （β-CD-B2 ） as chiral additive in high performance capillary electrophoresis （HPCE）. The effect of concentration and pH of borax buffer solution, concentration of chiral additive β-CD-B2 and separation vohage on the separation were discussed. The results indicated that the HPCE resolution （ RS ） under optimum condition was 4.26 in 8 min . Linear range of ibuprofen enantiomers＇ s separated front peaks is 0. 053 - 0. 942g/L, while later peak linear range is 0. 178 -0.942g/L. Thus ibuprofen and bupivacaine, two chiral drugs can be separated under the same condition best suited for ibuprofen.