纤维素科学与技术
纖維素科學與技術
섬유소과학여기술
JOURNAL OF CELLULOSE SCIENCE AND TECHNOLOGY
2012年
2期
1-10
,共10页
曹杰%张帅%杨林峰%王旺霞%吴文娟%金永灿
曹傑%張帥%楊林峰%王旺霞%吳文娟%金永燦
조걸%장수%양림봉%왕왕하%오문연%금영찬
稻草%Na2SO3预处理%化学成分%酶水解%糖转化率
稻草%Na2SO3預處理%化學成分%酶水解%糖轉化率
도초%Na2SO3예처리%화학성분%매수해%당전화솔
rice straw%sodium sulfite pretreatment%chemical composition%enzymatic hydrolysis%sugar conversion
研究了亚硫酸钠预处理对稻草化学组分变化及酶水解性能的影响。结果表明,提高温度或增加Na2SO3用量可以脱除更多的木质素和半纤维素,酶水解效率也相应提高,但木质素脱除率达到50%以后,继续增强预处理条件,对酶水解糖得率无显著的促进作用。相比而言,加大Na2SO3用量更有利于使木质素溶出,提高温度更有利于使高聚糖溶出,加大Na2SO3用量比提高温度对酶水解效率的提高影响更显著。通过实验得到亚硫酸钠预处理稻草的最优条件,在温度为140℃,Na2SO3用量为16%,纤维素酶用量为20 FPU/g(对纤维素)时,总糖转化率达到最大,为74.9%,此时的总糖得率为43.5%。
研究瞭亞硫痠鈉預處理對稻草化學組分變化及酶水解性能的影響。結果錶明,提高溫度或增加Na2SO3用量可以脫除更多的木質素和半纖維素,酶水解效率也相應提高,但木質素脫除率達到50%以後,繼續增彊預處理條件,對酶水解糖得率無顯著的促進作用。相比而言,加大Na2SO3用量更有利于使木質素溶齣,提高溫度更有利于使高聚糖溶齣,加大Na2SO3用量比提高溫度對酶水解效率的提高影響更顯著。通過實驗得到亞硫痠鈉預處理稻草的最優條件,在溫度為140℃,Na2SO3用量為16%,纖維素酶用量為20 FPU/g(對纖維素)時,總糖轉化率達到最大,為74.9%,此時的總糖得率為43.5%。
연구료아류산납예처리대도초화학조분변화급매수해성능적영향。결과표명,제고온도혹증가Na2SO3용량가이탈제경다적목질소화반섬유소,매수해효솔야상응제고,단목질소탈제솔체도50%이후,계속증강예처리조건,대매수해당득솔무현저적촉진작용。상비이언,가대Na2SO3용량경유리우사목질소용출,제고온도경유리우사고취당용출,가대Na2SO3용량비제고온도대매수해효솔적제고영향경현저。통과실험득도아류산납예처리도초적최우조건,재온도위140℃,Na2SO3용량위16%,섬유소매용량위20 FPU/g(대섬유소)시,총당전화솔체도최대,위74.9%,차시적총당득솔위43.5%。
The effects of sodium sulfite (Na2SO3) pretreatment on chemical composision and enzymatic hydrolysis of rice straw were investigated. Raising the temperature or increasing the charge of sodium sulfite can improve the dissolution of lignin and saccharification efficiency, while most carbohydrate is retained. However, when the dissolution of lignin attained 40%, the improvement of saccharification efficiency was nonsignificant. After enzymatic hydrolysis, total sugar conversion rate of 74.9% and total sugar yield rate of 43.5% can be achieved by using the substrate pretreated under the condition of temperature 140~C, sodium sulfite charge 16% and cellulase amount 20 FPU/g (cellulose).