CAJ | 학술논문

为研究5-aza—dC和TSA提高核移植胚胎体外发育能力的作用是否有供体细胞特异性，实验选择皮肤成纤维细胞、胎儿成纤维细胞和卵丘颗粒细胞作为核供体细胞进行核移植，联合使用20nM（72h）5-aza—dc和50nM（12h）TSA处理供体细胞和重构胚，比较5-ttZa—dC＋TSA对不同供体细胞来源核移植胚胎体外发育的影响。实验结果表明，经5-azaLdC＋TSA处理后，极显著提高了3种供体细胞来源核移植胚胎的体外囊胚发育率（P〈0．01）。虽然胎儿成纤维细胞和卵丘颗粒细胞得到的发育率稍高于皮肤成纤维细胞组，但各对照组之间以及各处理组之间的体外发育率没有明显差异，5-aza—dC和TSA对核移植胚胎体外发育的影响没有供体细胞特异性。
위연구5-aza—dC화TSA제고핵이식배태체외발육능력적작용시부유공체세포특이성，실험선택피부성섬유세포、태인성섬유세포화란구과립세포작위핵공체세포진행핵이식，연합사용20nM（72h）5-aza—dc화50nM（12h）TSA처리공체세포화중구배，비교5-ttZa—dC＋TSA대불동공체세포래원핵이식배태체외발육적영향。실험결과표명，경5-azaLdC＋TSA처리후，겁현저제고료3충공체세포래원핵이식배태적체외낭배발육솔（P〈0．01）。수연태인성섬유세포화란구과립세포득도적발육솔초고우피부성섬유세포조，단각대조조지간이급각처리조지간적체외발육솔몰유명현차이，5-aza—dC화TSA대핵이식배태체외발육적영향몰유공체세포특이성。
To investigate the effects of 5-aza-dC and TSA on the development of embryos cloned from different donor cells, the fetal fibroblast ceils, adult skin fibroblast cells, and cumulus cells were used as donor cells for nuclear transfer, the donor cells and cloned embryos were treated with TSA （50 nM, 12 h） and 5-aza-dC （20 nM,72 h） , and the effects of these reagents on the development of cloned embryos were investigated. The results show that 5-aza-dC combined with TSA could significantly promoted the development potential of cloned embryos （P〈0.01） , and the beneficial effects of TSA and 5-aza-dC im cloned embryos development were not of specific characteristics for the donor cells.