CAJ | 학술논문

以甘蔗品种FN28为材料,首次克隆甘蔗ShSUT4基因。测序结果表明,该基因约为4.8 kb,包含5个外显子和4个内含子,并包括完整的开放阅读框。该基因5＇侧翼序列长度约为1.8 kb。采用PLACE、PlantCARE在线启动子预测工具分析表明,该序列含有启动子的特定结构,如TATA-box、CAAT-box等,还含有一些顺式作用元件如光响应元件、MYB结合位点、ABRE响应元件等,表明甘蔗ShSUT4基因的表达可能受光照、MYB和ABRE等的调控。
이감자품충FN28위재료,수차극륭감자ShSUT4기인。측서결과표명,해기인약위4.8 kb,포함5개외현자화4개내함자,병포괄완정적개방열독광。해기인5＇측익서렬장도약위1.8 kb。채용PLACE、PlantCARE재선계동자예측공구분석표명,해서렬함유계동자적특정결구,여TATA-box、CAAT-box등,환함유일사순식작용원건여광향응원건、MYB결합위점、ABRE향응원건등,표명감자ShSUT4기인적표체가능수광조、MYB화ABRE등적조공。
A DNA encoding ShSUT4 was isolated from the leaf of sugarcane.The sequence had 5 exons and 4 introns with a size of 4.8 kb and included a whole open reading frame.The 5′ flanking sequence of ShSUT4 was about 1.8 kb.Based on the analysis of PLACE and PlantCARE,TATA-box,CAAT-box and some cis-acting elements such as light-induced responsive elements,MYB binding site,ABRE responsive were predicted in promoter sequence.So it implies that the ShSUT4 gene expression involved light,MYB,ABRE.