CAJ | 학술논문

本试验以建立的南林95杨高频再生体系为基础，利用正交试验设计，通过GUS组织染色分析法研究了预培养时间、茵液浓度、As浓度、侵染时间和共培养时间5个因素在4个水平上对南林95杨遗传转化的影响，并探讨了南林95杨转化中添加卡那霉素的适宜浓度。运用DPS软件对试验结果进行分析，建立了杨树遗传转化体系。结果表明：外植体预培养7d，在含As200μmol／L的茵液浓度为0．6的农杆菌液中侵染20rain，共培养3d为最佳遗传转化体系，适宜的卡那霉素筛选浓度为50mg／L；本试验共获得6株转化植株，经GUS染色检测和PCR分析表明．GUS基因已初步整合进入南林95杨基因组中。
본시험이건립적남림95양고빈재생체계위기출，이용정교시험설계，통과GUS조직염색분석법연구료예배양시간、인액농도、As농도、침염시간화공배양시간5개인소재4개수평상대남림95양유전전화적영향，병탐토료남림95양전화중첨가잡나매소적괄의농도。운용DPS연건대시험결과진행분석，건립료양수유전전화체계。결과표명：외식체예배양7d，재함As200μmol／L적인액농도위0．6적농간균액중침염20rain，공배양3d위최가유전전화체계，괄의적잡나매소사선농도위50mg／L；본시험공획득6주전화식주，경GUS염색검측화PCR분석표명．GUS기인이초보정합진입남림95양기인조중。
Based on the established regeneration system of poplar Nanlin 95 （Populus deltoids Bartr. cv. Lux x P. euramericana （Dode）Guineir cv. 1-45 / 51）, the effects of the preculture period, concentrations of bacterium solution, AS concentrations, infection time and co-culture period on the genetic transformation of the poplar variety at 4 levels were studied with orthogonal experiment design and GUS tissue coloration analysis, and adding an optimal concentration of kanamycin in the genetic transformation of the poplar variety was also probed. The experimental data were analyzed with DPS software and a genetic transformation system for poplar was established. The results showed that the optimum genetic transformation system could be achieved with 7-day preculture, 20-minute infection of Agrobacterium （OD6oo = 0.6, 200 μmol/L AS） and 3-day co culture. The selected optimal concentration of kanamycin was 50 mg/L. 6 transgenetic plants were obtained in the experiment and preliminary integration of GUS genes in these poplar Nanhn 95 plants were confirmed by GUS coloration analysis and PCR tests.