广西植物
廣西植物
엄서식물
GUIHAIA
2013年
6期
878-882,800
,共6页
李晓霞%沈奕德%范志伟%黄乔乔%程汉亭%刘丽珍
李曉霞%瀋奕德%範誌偉%黃喬喬%程漢亭%劉麗珍
리효하%침혁덕%범지위%황교교%정한정%류려진
肿柄菊%挥发油%化感物质%化感作用
腫柄菊%揮髮油%化感物質%化感作用
종병국%휘발유%화감물질%화감작용
Tithonia diversifolia%volatile oil%allelopathy%allelochemicals
采用水蒸气蒸馏法提取肿柄菊地上部分的挥发油，利用 GC-MS 技术对肿柄菊地上部分的挥发油进行分析，以假臭草、巴西含羞草、含羞草、蝶豆和鬼针草5种肿柄菊伴生植物为供试植物，用半密闭容器法对肿柄菊挥发油的化感效应进行生物测定.GC-MS分析结果显示，从肿柄菊挥发油中鉴定出50个化合物，占总出峰面积的94．79％，肿柄菊挥发油中主要含有α-蒎烯(63．81％)、柠檬烯(7．07％)、β-石竹烯(4．85％)、双环大香烯(2．95％)、香桧烯(2．78％)、斯巴醇(2．70％)等萜类和醇类化合物，占挥发油总量的94．15％，其中萜类化合物含量占总挥发油的86．01％.生测结果显示，肿柄菊挥发油对5种受试植物的种子萌发和幼苗生长均产生抑制作用，其中对巴西含羞草的抑制作用最为显著，而对含羞草和蝶豆影响较小.说明肿柄菊挥发油在其入侵扩散中发挥一定的化感作用.
採用水蒸氣蒸餾法提取腫柄菊地上部分的揮髮油，利用 GC-MS 技術對腫柄菊地上部分的揮髮油進行分析，以假臭草、巴西含羞草、含羞草、蝶豆和鬼針草5種腫柄菊伴生植物為供試植物，用半密閉容器法對腫柄菊揮髮油的化感效應進行生物測定.GC-MS分析結果顯示，從腫柄菊揮髮油中鑒定齣50箇化閤物，佔總齣峰麵積的94．79％，腫柄菊揮髮油中主要含有α-蒎烯(63．81％)、檸檬烯(7．07％)、β-石竹烯(4．85％)、雙環大香烯(2．95％)、香檜烯(2．78％)、斯巴醇(2．70％)等萜類和醇類化閤物，佔揮髮油總量的94．15％，其中萜類化閤物含量佔總揮髮油的86．01％.生測結果顯示，腫柄菊揮髮油對5種受試植物的種子萌髮和幼苗生長均產生抑製作用，其中對巴西含羞草的抑製作用最為顯著，而對含羞草和蝶豆影響較小.說明腫柄菊揮髮油在其入侵擴散中髮揮一定的化感作用.
채용수증기증류법제취종병국지상부분적휘발유，이용 GC-MS 기술대종병국지상부분적휘발유진행분석，이가취초、파서함수초、함수초、접두화귀침초5충종병국반생식물위공시식물，용반밀폐용기법대종병국휘발유적화감효응진행생물측정.GC-MS분석결과현시，종종병국휘발유중감정출50개화합물，점총출봉면적적94．79％，종병국휘발유중주요함유α-파희(63．81％)、저몽희(7．07％)、β-석죽희(4．85％)、쌍배대향희(2．95％)、향회희(2．78％)、사파순(2．70％)등첩류화순류화합물，점휘발유총량적94．15％，기중첩류화합물함량점총휘발유적86．01％.생측결과현시，종병국휘발유대5충수시식물적충자맹발화유묘생장균산생억제작용，기중대파서함수초적억제작용최위현저，이대함수초화접두영향교소.설명종병국휘발유재기입침확산중발휘일정적화감작용.
Volatile oil was obtained from above ground part of Tithonia diversifolia by steam distillation method,and the main chemical components in the volatile oil were identified by gas chromatography-mass.Allelopathy of volatile oil from T.diversifolia on seed germination and seedling growth of 5 co-occuring plant species,Eupatorium catari-um,Mimosa diplotrich,Mimosa pudica,Clitoria ternatea,Bidens pilosa was studied in a semi-closed container.50 compounds were identified,accounting to 94.79% of the total detected constituents.The main components wereα-Pi-nene(63.81%),Limonene(7.07%),β-Caryophyllene(4.85%),Bicyclogermacrene(2.95%),Sabinene(2.78%),and Spathulenol(2.702%),which accounted for 94.15%of the total amount of the essential oil samples.Terpenes accoun-ted for 86.01% of the total.The bioassay test results demonstrated that volatile oil of T.diversifolia could inhibit the seed germination and growth of all tested plant.The inhibitory effects on Mimosa diplotricha was the largest, while Mimosa pudica and Clitoria ternatea were relatively small.These results indicated that volatile oil of T.diver-sifolia played a certain alllopathy invasive in invasion of diffusion.
