CAJ | 학술논문

红树植物杯萼海桑是最耐盐的红树植物之一.S-腺苷甲硫氨酸合成酶(S-adenosylmethionine syn-thetase,SAMS)是 S-腺苷甲硫氨酸(S-adenosylmethionine,SAM)生物合成途径的关键酶.SAMS 作为一个逆境胁迫响应蛋白在植物的耐盐调控中发挥着极其重要的作用.本文结合杯萼海桑根的转录组注释,根据编码区序列设计引物,通过PCR克隆杯萼海桑SAMS 基因的编码区 cDNA,并对其进行生物信息分析,为研究杯萼海桑适应逆境的机制奠定理论基础.结果显示PCR扩增了一个长1182 bp 的基因片段,该片段编码由393个氨基酸组成的S-腺苷甲硫氨酸合成酶.同源性比对及进化树分析显示杯萼海桑的SAMS 氨基酸序列进化上相对保守.本研究首次从红树林植物杯萼海桑中克隆S-腺苷甲硫氨酸合成酶基因,并获得其编码区序列,为进一步研究杯萼海桑应对逆境胁迫的分子生物学机制与胁迫相关基因调控网络奠定基础.
홍수식물배악해상시최내염적홍수식물지일.S-선감갑류안산합성매(S-adenosylmethionine syn-thetase,SAMS)시 S-선감갑류안산(S-adenosylmethionine,SAM)생물합성도경적관건매.SAMS 작위일개역경협박향응단백재식물적내염조공중발휘착겁기중요적작용.본문결합배악해상근적전록조주석,근거편마구서렬설계인물,통과PCR극륭배악해상SAMS 기인적편마구 cDNA,병대기진행생물신식분석,위연구배악해상괄응역경적궤제전정이론기출.결과현시PCR확증료일개장1182 bp 적기인편단,해편단편마유393개안기산조성적S-선감갑류안산합성매.동원성비대급진화수분석현시배악해상적SAMS 안기산서렬진화상상대보수.본연구수차종홍수림식물배악해상중극륭S-선감갑류안산합성매기인,병획득기편마구서렬,위진일보연구배악해상응대역경협박적분자생물학궤제여협박상관기인조공망락전정기출.
Sonneratia alba is one of the most salt-tolerant mangroves.S-adenosylmethionine synthetase(SAMS)which was in response to abiotic stresses played an important role in salt tolerance.In order to study the biological mecha-nism of S.alba for adapting stress,the coding region of SaSAMS gene which codes a key enzyme in the S-adenosyl-methionine(SAM)biosynthetic pathway was isolated.Based on the annotation of root transcriptome of S.alba,prim-ers was designed and cDNA of SaSAMS gene was amplified by PCR.The complete coding sequence of SaSAMS gene was 1 182 bp and it encoded a protein of 393 amino acids.Homology comparison and evolutionary tree analysis showed that the SAMS amino acid sequences were relatively conserved in plants.The SaSAMS gene was cloned from a mangrove tree S.alba for the first time and this would facilitate further investigation on molecular mechanism of stress tolerance and regulation networks of stress-related genes in S.alba.